Kanclerski K, Granström M, Möllby R
National Bacteriological Laboratory, Stockholm, Sweden.
Acta Pathol Microbiol Immunol Scand B. 1987 Aug;95(4):241-4. doi: 10.1111/j.1699-0463.1987.tb03119.x.
The immunological relation between serum antibodies to pneumolysin and to streptolysin O was studied in patients with pneumococcal pneumonia (n = 40), patients with infections due to beta-haemolytic streptococci (n = 35), healthy human controls (n = 60) and in rabbits immunized with pneumolysin. There was no correlation between anti-pneumolysin and anti-streptolysin O titers (r = -0.279). The distribution of anti-pneumolysin titers in patients with high anti-streptolysin O titers did not differ from healthy controls. However, there was a tendency to increased or rising anti-streptolysin O titers in patients with pneumococcal infection. Antibodies obtained during pneumococcal infection might thus give false-positive reactions in the streptolysin O neutralization test. Serum antibodies to streptolysin O do not cross-react with pneumolysin in an ELISA. The pneumolysin ELISA for detection of pneumococcal disease will therefore not be disturbed by false-positive reactions due to antibodies directed against beta-haemolytic streptococci.
在肺炎球菌肺炎患者（n = 40）、β溶血性链球菌感染患者（n = 35）、健康人对照组（n = 60）以及用肺炎溶血素免疫的兔子中，研究了抗肺炎溶血素血清抗体与抗链球菌溶血素O之间的免疫关系。抗肺炎溶血素和抗链球菌溶血素O滴度之间无相关性（r = -0.279）。抗链球菌溶血素O滴度高的患者中抗肺炎溶血素滴度的分布与健康对照组无差异。然而，肺炎球菌感染患者有抗链球菌溶血素O滴度升高的趋势。因此，肺炎球菌感染期间获得的抗体可能在链球菌溶血素O中和试验中产生假阳性反应。在酶联免疫吸附测定中，抗链球菌溶血素O血清抗体与肺炎溶血素不发生交叉反应。因此，用于检测肺炎球菌疾病的肺炎溶血素酶联免疫吸附测定不会因针对β溶血性链球菌的抗体而受到假阳性反应的干扰。
