Jalonen E, Paton J C, Koskela M, Kerttula Y, Leinonen M
National Public Health Institute, Helsinki, Finland.
J Infect. 1989 Sep;19(2):127-34. doi: 10.1016/s0163-4453(89)91864-1.
An enzymeimmunoassay (EIA) for measuring antibodies to pneumococcal pneumolysin has been developed. The method was used to study the possible pneumococcal aetiology of pneumonia in 159 mostly elderly patients admitted to hospital because of a positive chest X-ray. The results obtained with the assay were compared to those obtained by other diagnostic methods, namely blood culture, detection of pneumococcal antigen in urine and demonstration of an antibody response to pneumococcal C-polysaccharide and capsular polysaccharides. Antibody response to pneumolysin was found in 32 of 39 (82%) patients with pneumococcal pneumonia aetiologically diagnosed presumptively by other methods. In addition, the EIA for pneumolysin antibodies was positive in 31 patients without evidence of pneumococcal aetiology by other methods. The clinical and laboratory investigations of these patients supported the presumption of bacterial infection. We conclude that the EIA we have developed for measuring pneumolysin antibodies is a promising, sensitive method for the presumptive aetiological diagnosis of pneumococcal pneumonia. The assay is simple to perform because only one antigen is needed and measurement of IgG antibodies alone seems to be enough for aetiological diagnosis.
已开发出一种用于检测抗肺炎球菌溶血素抗体的酶免疫测定法（EIA）。该方法用于研究159名因胸部X线检查呈阳性而入院的大多为老年患者肺炎可能的肺炎球菌病因。将该测定法获得的结果与通过其他诊断方法获得的结果进行比较，其他诊断方法即血培养、尿液中肺炎球菌抗原的检测以及对肺炎球菌C多糖和荚膜多糖抗体反应的证明。在通过其他方法初步病因诊断为肺炎球菌肺炎的39名患者中的32名（82%）发现了对溶血素的抗体反应。此外，在其他方法未显示肺炎球菌病因证据的31名患者中，溶血素抗体的EIA呈阳性。这些患者的临床和实验室检查支持细菌感染的推测。我们得出结论，我们开发的用于检测溶血素抗体的EIA是一种有前景的、敏感的方法，用于肺炎球菌肺炎的初步病因诊断。该测定法操作简单，因为仅需要一种抗原，而且仅检测IgG抗体似乎就足以进行病因诊断。
