利用合成肽对肺炎球菌溶血素免疫反应位点的定位
Mapping of immunoreactive sites of pneumococcal pneumolysin by use of synthetic peptides.
作者信息
Salo P, Närvänen A, Leinonen M
机构信息
National Public Health Institute, Helsinki, Finland.
出版信息
Infect Immun. 1993 Jul;61(7):2822-6. doi: 10.1128/iai.61.7.2822-2826.1993.
Abstract
In the search for better diagnostic tools and improved vaccines against pneumococcal diseases, continuous antigenic sites of the pneumococcal pneumolysin molecule were identified with partially overlapping synthetic peptides. Peptides were derived from the predicted amino acid sequence according to the known DNA sequence of pneumolysin. The immunoreactivities of the peptides were studied by enzyme immunoassay with seven human serum samples and three rabbit hyperimmune serum samples. Two immunoreactive sites in the overlapping peptides at the amino-terminal end of the pneumolysin molecule were identified in this study. Two common immunoreactive sites for both human and rabbit sera were demonstrated.
摘要
在寻找针对肺炎球菌疾病的更好诊断工具和改进疫苗的过程中，利用部分重叠的合成肽鉴定了肺炎球菌溶血素分子的连续抗原位点。根据肺炎球菌溶血素已知的DNA序列，从预测的氨基酸序列中推导肽段。通过酶免疫测定法，使用7份人血清样本和3份兔超免疫血清样本研究了这些肽段的免疫反应性。本研究在肺炎球菌溶血素分子氨基末端的重叠肽段中鉴定出两个免疫反应位点。证实了人和兔血清存在两个共同的免疫反应位点。
相似文献
1
Mapping of immunoreactive sites of pneumococcal pneumolysin by use of synthetic peptides.利用合成肽对肺炎球菌溶血素免疫反应位点的定位
Infect Immun. 1993 Jul;61(7):2822-6. doi: 10.1128/iai.61.7.2822-2826.1993.
2
Complement activation and antibody binding by pneumolysin via a region of the toxin homologous to a human acute-phase protein.肺炎溶血素通过毒素中与人类急性期蛋白同源的区域激活补体并结合抗体。
Mol Microbiol. 1991 Aug;5(8):1883-8. doi: 10.1111/j.1365-2958.1991.tb00812.x.
3
Immunological relation between serum antibodies against pneumolysin and against streptolysin O.抗肺炎球菌溶血素血清抗体与抗链球菌溶血素O之间的免疫关系。
Acta Pathol Microbiol Immunol Scand B. 1987 Aug;95(4):241-4. doi: 10.1111/j.1699-0463.1987.tb03119.x.
4
Nasopharyngeal antibodies to pneumococcal pneumolysin in children with acute otitis media.急性中耳炎患儿中针对肺炎球菌溶血素的鼻咽部抗体
Clin Diagn Lab Immunol. 1995 Nov;2(6):704-7. doi: 10.1128/cdli.2.6.704-707.1995.
5
Antibodies to pneumolysin and pneumococcal capsular polysaccharides in middle ear fluid of children with acute otitis media.急性中耳炎患儿中耳积液中肺炎球菌溶血素及肺炎球菌荚膜多糖抗体
Acta Otolaryngol. 1995 Nov;115(6):796-803. doi: 10.3109/00016489509139404.
6
Expression of the pneumolysin gene in Escherichia coli: rapid purification and biological properties.
Biochim Biophys Acta. 1989 Jan 23;1007(1):67-72. doi: 10.1016/0167-4781(89)90131-0.
7
Comparative efficacy of pneumococcal neuraminidase and pneumolysin as immunogens protective against Streptococcus pneumoniae.
Microb Pathog. 1988 Dec;5(6):461-7. doi: 10.1016/0882-4010(88)90007-1.
8
Genetic diversity and antigenicity analysis of Streptococcus pneumoniae pneumolysin isolated from children with pneumococcal infection.肺炎链球菌溶血素的遗传多样性和抗原性分析，该菌分离自肺炎链球菌感染患儿。
Int J Infect Dis. 2019 Sep;86:57-64. doi: 10.1016/j.ijid.2019.06.025. Epub 2019 Jun 27.
9
Immunodetection of pneumolysin in human urine by ELISA.通过酶联免疫吸附测定法对人尿液中的肺炎溶血素进行免疫检测。
J Microbiol Methods. 2003 Jul;54(1):47-55. doi: 10.1016/s0167-7012(03)00004-6.
10
Characterisation of mouse monoclonal antibodies for pneumolysin: fine epitope mapping and V gene usage.肺炎溶血素小鼠单克隆抗体的特性：精细表位图谱分析及V基因使用情况
Immunol Lett. 2003 Sep 8;88(3):227-39. doi: 10.1016/s0165-2478(03)00081-6.
引用本文的文献
1
Crucial role of perfringolysin O D1 domain in orchestrating structural transitions leading to membrane-perforating pores: a hydrogen-deuterium exchange study.产气荚膜梭菌溶血素O D1结构域在引发导致膜穿孔孔道的结构转变中的关键作用：一项氢-氘交换研究
J Biol Chem. 2014 Oct 10;289(41):28738-52. doi: 10.1074/jbc.M114.577981. Epub 2014 Aug 27.
2
Construction and immunological characterization of a novel nontoxic protective pneumolysin mutant for use in future pneumococcal vaccines.一种新型无毒保护性肺炎溶血素突变体的构建及其免疫学特性研究，用于未来的肺炎球菌疫苗。
Infect Immun. 2006 Jan;74(1):586-93. doi: 10.1128/IAI.74.1.586-593.2006.
3
Molecular cloning, sequencing, expression, and characterization of an immunogenic 43-kilodalton lipoprotein of Bartonella bacilliformis that has homology to NlpD/LppB.与NlpD/LppB具有同源性的杆状巴尔通体43千道尔顿免疫原性脂蛋白的分子克隆、测序、表达及特性分析
Infect Immun. 2000 Sep;68(9):4972-9. doi: 10.1128/IAI.68.9.4972-4979.2000.
4
Analysis of the Yersinia pestis V protein for the presence of linear antibody epitopes.分析鼠疫耶尔森菌V蛋白中线性抗体表位的存在情况。
Infect Immun. 1998 Feb;66(2):521-7. doi: 10.1128/IAI.66.2.521-527.1998.
5
Nasopharyngeal antibodies to pneumococcal pneumolysin in children with acute otitis media.急性中耳炎患儿中针对肺炎球菌溶血素的鼻咽部抗体
Clin Diagn Lab Immunol. 1995 Nov;2(6):704-7. doi: 10.1128/cdli.2.6.704-707.1995.
6
Functional analysis of pneumolysin by use of monoclonal antibodies.利用单克隆抗体对肺炎溶血素进行功能分析。
Infect Immun. 1996 Feb;64(2):480-4. doi: 10.1128/iai.64.2.480-484.1996.
本文引用的文献
1
Inhibition of human polymorphonuclear leukocyte respiratory burst, bactericidal activity, and migration by pneumolysin.肺炎溶血素对人多形核白细胞呼吸爆发、杀菌活性及迁移的抑制作用。
Infect Immun. 1983 Sep;41(3):1212-6. doi: 10.1128/iai.41.3.1212-1216.1983.
2
Effect of immunization with pneumolysin on survival time of mice challenged with Streptococcus pneumoniae.用肺炎溶血素免疫对肺炎链球菌攻击的小鼠存活时间的影响。
Infect Immun. 1983 May;40(2):548-52. doi: 10.1128/iai.40.2.548-552.1983.
3
Activation of human complement by the pneumococcal toxin pneumolysin.肺炎球菌毒素肺炎溶血素对人补体的激活作用。
Infect Immun. 1984 Mar;43(3):1085-7. doi: 10.1128/iai.43.3.1085-1087.1984.
4
Inhibition of in vitro human lymphocyte response by the pneumococcal toxin pneumolysin.肺炎球菌毒素肺炎溶血素对体外人淋巴细胞反应的抑制作用。
Infect Immun. 1984 Nov;46(2):585-9. doi: 10.1128/iai.46.2.585-589.1984.
5
Secretion of Escherichia coli beta-lactamase from Bacillus subtilis by the aid of alpha-amylase signal sequence.借助α-淀粉酶信号序列从枯草芽孢杆菌中分泌大肠杆菌β-内酰胺酶。
Proc Natl Acad Sci U S A. 1982 Sep;79(18):5582-6. doi: 10.1073/pnas.79.18.5582.
6
The aetiology of pneumonia. Application of bacterial serology and basic laboratory methods.
J Infect. 1987 Jan;14(1):21-30. doi: 10.1016/s0163-4453(87)90730-4.
7
Diagnosis of pneumococcal pneumonia by enzyme-linked immunosorbent assay of antibodies to pneumococcal hemolysin (pneumolysin).通过酶联免疫吸附测定肺炎球菌溶血素（肺炎溶素）抗体来诊断肺炎球菌肺炎。
J Clin Microbiol. 1987 Feb;25(2):226-9. doi: 10.1128/jcm.25.2.226-229.1987.
8
Immunological relation between serum antibodies against pneumolysin and against streptolysin O.抗肺炎球菌溶血素血清抗体与抗链球菌溶血素O之间的免疫关系。
Acta Pathol Microbiol Immunol Scand B. 1987 Aug;95(4):241-4. doi: 10.1111/j.1699-0463.1987.tb03119.x.
9
Molecular cloning, characterization, and complete nucleotide sequence of the gene for pneumolysin, the sulfhydryl-activated toxin of Streptococcus pneumoniae.肺炎链球菌巯基激活毒素——肺炎溶血素基因的分子克隆、特性鉴定及全核苷酸序列分析
Infect Immun. 1987 May;55(5):1184-9. doi: 10.1128/iai.55.5.1184-1189.1987.
10
Nucleotide sequence of the streptolysin O (SLO) gene: structural homologies between SLO and other membrane-damaging, thiol-activated toxins.链球菌溶血素O（SLO）基因的核苷酸序列：SLO与其他膜损伤性硫醇激活毒素之间的结构同源性
Infect Immun. 1987 Dec;55(12):3228-32. doi: 10.1128/iai.55.12.3228-3232.1987.
Zotero 插件