Salo P, Närvänen A, Leinonen M
National Public Health Institute, Helsinki, Finland.
Infect Immun. 1993 Jul;61(7):2822-6. doi: 10.1128/iai.61.7.2822-2826.1993.
In the search for better diagnostic tools and improved vaccines against pneumococcal diseases, continuous antigenic sites of the pneumococcal pneumolysin molecule were identified with partially overlapping synthetic peptides. Peptides were derived from the predicted amino acid sequence according to the known DNA sequence of pneumolysin. The immunoreactivities of the peptides were studied by enzyme immunoassay with seven human serum samples and three rabbit hyperimmune serum samples. Two immunoreactive sites in the overlapping peptides at the amino-terminal end of the pneumolysin molecule were identified in this study. Two common immunoreactive sites for both human and rabbit sera were demonstrated.
在寻找针对肺炎球菌疾病的更好诊断工具和改进疫苗的过程中，利用部分重叠的合成肽鉴定了肺炎球菌溶血素分子的连续抗原位点。根据肺炎球菌溶血素已知的DNA序列，从预测的氨基酸序列中推导肽段。通过酶免疫测定法，使用7份人血清样本和3份兔超免疫血清样本研究了这些肽段的免疫反应性。本研究在肺炎球菌溶血素分子氨基末端的重叠肽段中鉴定出两个免疫反应位点。证实了人和兔血清存在两个共同的免疫反应位点。
