Sales Suiani da Silva, Dionísio Ana Paula, Adriano Lia Silveira, Melo Bárbara Rebeca Cordeiro de, Abreu Fernando Antônio Pinto de, Sampaio Helena Alves de Carvalho, Silva Ismael Dale Cotrim Guerreiro da, Carioca Antonio Augusto Ferreira
Postgraduate Program in Nutrition and Health, State University of Ceará, Fortaleza, Brazil.
Embrapa Agroindústria Tropical, Fortaleza, Brazil.
Nutrition. 2023 May;109:111948. doi: 10.1016/j.nut.2022.111948. Epub 2022 Dec 28.
The use of prebiotics has an effect on postprandial glucose and insulin concentrations; however, the interaction between the previous profile of the intestinal microbiota and the effect of supplementation with prebiotics is not clear. Our objective was to evaluate the effect of previous intestinal microbiota profile on the postprandial insulin response to yacon syrup, used as a source of fructooligosaccharides (FOSs), in young women. The product presents high levels of FOS.
In this double-blind, crossover, randomized clinical trial, 40 adult women were allocated to receive a breakfast containing 40 g of yacon syrup (14 g FOS, intervention A) or a breakfast containing 40 g of placebo (intervention B). On each intervention day, after 12 h of fasting, an aliquot of blood was collected for insulin analysis at 0, 15, 30, 45, 60, 90, and 120 min. The fecal sample was collected before the beginning of the interventions, and the DNA was extracted and quantified, with subsequent amplification of the 16S region, next-generation sequencing, and analysis of sequencing data.
The glucose and insulin concentrations were reduced after ingestion of yacon syrup compared with placebo, specifically at the 30 min to insulin. After the receiver operating characteristic analysis, six volunteers who did not respond to the yacon consumption intervention were identified. The abundance of the phylum Actinobacteria (P = 0.021) and the order Bifidobacteriales (P = 0.013) were positively associated with better insulin response. Other main phyla were not associated with intervention response.
The previous profile of the intestinal microbiota has an effect on the postprandial insulin response to FOSs, mainly in the phylum Actinobacteria and Bifidobacteriales order.
益生元的使用对餐后血糖和胰岛素浓度有影响；然而，肠道微生物群先前的状态与补充益生元的效果之间的相互作用尚不清楚。我们的目的是评估年轻女性先前的肠道微生物群状态对食用含有低聚果糖（FOS）的雪莲果糖浆后餐后胰岛素反应的影响。该产品含有高水平的FOS。
在这项双盲、交叉、随机临床试验中，40名成年女性被分配接受一份含有40克雪莲果糖浆（14克FOS，干预A）的早餐或一份含有40克安慰剂的早餐（干预B）。在每个干预日，禁食12小时后，在0、15、30、45、60、90和120分钟采集一份血液样本进行胰岛素分析。在干预开始前采集粪便样本，提取DNA并进行定量，随后扩增16S区域、进行下一代测序并分析测序数据。
与安慰剂相比，摄入雪莲果糖浆后葡萄糖和胰岛素浓度降低，特别是在30分钟时胰岛素浓度降低。经过受试者工作特征分析，确定了6名对食用雪莲果干预无反应的志愿者。放线菌门（P = 0.021）和双歧杆菌目（P = 0.013）的丰度与更好的胰岛素反应呈正相关。其他主要门类与干预反应无关。
肠道微生物群先前的状态对FOS的餐后胰岛素反应有影响，主要体现在放线菌门和双歧杆菌目。
