Department of Plant Biotechnology, Korea University, Seoul 02841, South Korea.
Institute of Life Science and Natural Resources, Korea University, Seoul 02841, South Korea.
Gene. 2023 Apr 15;860:147245. doi: 10.1016/j.gene.2023.147245. Epub 2023 Feb 1.
Common wheat (Triticum aestivum) is a major cereal crop grown and consumed globally. Recent advances in sequencing technology have facilitated the exploration of large and repetitive genomes. Plant sucrose transporter (SUT) genes are vital components of energy transport systems that play prominent roles in various plant functions, such as signaling and stress regulation. In this study, we identified and analyzed five novel sucrose transporter genes in wheat. The wheat sucrose transporter genes were divided into five clades based on their phylogenetic relationships. Synteny analysis revealed that synteny in the genome is highly conserved between wheat and rye, barley, and Brachypodium. Furthermore, the cis-element analysis indicated that sucrose transporter genes might be regulated by light and some phytohormone-related transcriptional factors. Overall, plant tissue-specific gene expression revealed enhanced expression of the transporter genes in the root and stem, whereas they were differentially expressed under abiotic stress treatments (cold, heat, NaCl, PEG-6000, and sucrose). These results indicate that each TaSUT gene may play a crucial role in stabilizing plants under stress by actively regulating the energy demands of cells. The findings of this study may provide a basis for further research on sucrose transporters and their significant roles in plant energy metabolism as well as in abiotic stress response, signaling, and regulation.
普通小麦（Triticum aestivum）是全球广泛种植和消费的主要粮食作物。测序技术的最新进展促进了对大型和重复基因组的探索。植物蔗糖转运蛋白（SUT）基因是能量运输系统的重要组成部分，在各种植物功能中发挥着重要作用，如信号转导和应激调节。在本研究中，我们在小麦中鉴定和分析了五个新的蔗糖转运蛋白基因。根据系统发育关系，小麦蔗糖转运蛋白基因分为五个分支。共线性分析表明，小麦和黑麦、大麦和拟南芥之间的基因组共线性高度保守。此外，顺式作用元件分析表明，蔗糖转运蛋白基因可能受到光和一些植物激素相关转录因子的调控。总的来说，植物组织特异性基因表达显示，转运蛋白基因在根和茎中表达增强，而在非生物胁迫处理（冷、热、NaCl、PEG-6000 和蔗糖）下则表现出差异表达。这些结果表明，每个 TaSUT 基因可能通过积极调节细胞的能量需求，在稳定植物应对胁迫方面发挥关键作用。本研究的结果可能为进一步研究蔗糖转运蛋白及其在植物能量代谢以及非生物胁迫响应、信号转导和调节中的重要作用提供基础。
