Center of Pharmaceutical Engineering and Technology, Harbin University of Commerce, Harbin 150076, PR China.
Center of Pharmaceutical Engineering and Technology, Harbin University of Commerce, Harbin 150076, PR China; Key Open Laboratory of Cold Water Fish Germplasm Resources and Breeding of Heilongjiang Province, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, PR China.
Int J Biol Macromol. 2023 Mar 31;232:123500. doi: 10.1016/j.ijbiomac.2023.123500. Epub 2023 Jan 31.
Lycium barbarum polysaccharide (LBP) is the main active component of Lycium barbarum (L. barbarum), which has important medicinal and nutritional value. However, the effect of LBP treatment on Luciobarbus capito (L. capito) still remains unknown. Given this, the current work aims to probe the underlying effect of different levels of LBP treatment (i.e. 0.10, 0.50 and 1.00 g/L) on L. capito in the context of enzymatic activity analysis, histological observations and gut microbiota analysis. Compared with control group, the activities of hepatic antioxidant enzymes, intestinal digestive enzymes and hepatic immune enzyme were found to be significantly increased after 0.10 g/L LBP and 0.50 g/L LBP treatment (P < 0.05). This result indicated that moderate levels of LBP treatment could dramatically enhance the immunity and antioxidant capacity of L. capito. Furthermore, the compositional structures of the gut microbiota in L. capito were found to be greatly shaped after LBP treatment, whereas the diversity and abundance of the gut microbiota were only found to be slightly changed (P > 0.05). No significant changes were screened in the morphologic structures of gut constructions. This work would provide theoretical and experimental basis for future application of LBP as supplement in the culture process of the farmed fish.
枸杞多糖（LBP）是枸杞（L. barbarum）的主要活性成分，具有重要的药用和营养价值。然而，LBP 处理对黄河鮈（L. capito）的影响尚不清楚。鉴于此，本研究旨在探究不同水平 LBP 处理（即 0.10、0.50 和 1.00 g/L）对黄河鮈的潜在影响，包括酶活性分析、组织学观察和肠道微生物群分析。与对照组相比，经 0.10 g/L 和 0.50 g/L LBP 处理后，黄河鮈的肝抗氧化酶、肠消化酶和肝免疫酶活性均显著升高（P < 0.05）。这一结果表明，适度水平的 LBP 处理可显著增强黄河鮈的免疫力和抗氧化能力。此外，LBP 处理后黄河鮈肠道微生物群的组成结构发生了很大变化，而肠道微生物群的多样性和丰度仅发生了轻微变化（P > 0.05）。肠道结构的形态结构未发生明显变化。本研究为未来将 LBP 作为养殖鱼类养殖过程中的补充剂的应用提供了理论和实验基础。
