Intrinsic STING Switches off Pathogenetic Programs of Th1 Cells to Inhibit Colitis.

BACKGROUND & AIMS: T helper 1 (Th1) effector cells are implicated in inflammatory bowel disease. The stimulator of interferon genes (STING), an intracellular DNA sensor, has been shown to regulate infection and various cancers. However, whether and how intrinsic STING signaling in Th1 cells regulates colitis is still unknown.

METHODS

Dextran sodium sulfate-induced colitis and wild-type/STING-deficient CD4T cell adoptive transfer models were used to analyze the role of STING in regulating colitis. The effect of STING on Th1 cells was determined by flow cytometry, RNA sequencing, metabolic assays, and mitochondrial functions. 16S ribosomal RNA sequencing and germ-free mice were used to investigate whether the microbiota were involved. The in vivo effect of STING agonist in murine colitis was determined. The expression and role of STING in human T cells were also determined.

RESULTS

Activation of STING transformed proinflammatory IFNγTh1 cells into IL-10IFNγTh1 cells, which were dramatically less pathogenic in inducing colitis. STING promoted Th1 interleukin (IL)-10 production by inducing STAT3 translocation into nuclear and mitochondria, which promoted Blimp1 expression and mitochondrial oxidation, respectively. Blockade of glucose or glutamine-derived oxidation, but not lipid-derived oxidation, suppressed STING induction of IL-10. Gut microbiota were changed in STING mice, but the altered microbiota did not mediate STING effects on intestinal CD4T cell production of IL-10. Translationally, STING agonists suppressed both acute and chronic colitis. Intestinal STING CD4T cells were increased in inflammatory bowel disease patients, and STING agonists upregulated IL-10 production in human CD4T cells.

CONCLUSIONS

These findings establish a crucial role of T cell-intrinsic STING in switching off the pathogenic programs of Th1 cells in intestinal inflammation.