Institut Curie, PSL Research University, CNRS UMR 3215, INSERM U934, F-75248 Paris Cedex 05, Paris, France.
Sorbonne Universités, UPMC Univ Paris 06, CNRS, CNRS UMR 3215, INSERM U934, F-75005, Paris, France.
Nat Commun. 2023 Feb 3;14(1):594. doi: 10.1038/s41467-023-36305-6.
Shape is a conspicuous and fundamental property of biological systems entailing the function of organs and tissues. While much emphasis has been put on how tissue tension and mechanical properties drive shape changes, whether and how a given tissue geometry influences subsequent morphogenesis remains poorly characterized. Here, we explored how curvature, a key descriptor of tissue geometry, impinges on the dynamics of epithelial tissue invagination. We found that the morphogenesis of the fold separating the adult Drosophila head and thorax segments is driven by the invagination of the Deformed (Dfd) homeotic compartment. Dfd controls invagination by modulating actomyosin organization and in-plane epithelial tension via the Tollo and Dystroglycan receptors. By experimentally introducing curvature heterogeneity within the homeotic compartment, we established that a curved tissue geometry converts the Dfd-dependent in-plane tension into an inward force driving folding. Accordingly, the interplay between in-plane tension and tissue curvature quantitatively explains the spatiotemporal folding dynamics. Collectively, our work highlights how genetic patterning and tissue geometry provide a simple design principle driving folding morphogenesis during development.
形状是生物系统的一个显著且基本的特性，它涉及器官和组织的功能。虽然人们非常重视组织张力和机械特性如何驱动形状变化，但给定的组织几何形状是否以及如何影响后续的形态发生仍然知之甚少。在这里，我们探讨了曲率（组织几何形状的一个关键描述符）如何影响上皮组织内陷的动力学。我们发现，分离成年果蝇头部和胸部节段的褶皱的形态发生是由变形（Dfd）同源异型区的内陷驱动的。Dfd 通过调节肌动球蛋白组织和平面上皮张力来控制内陷，这是通过 Toll 和 Dystroglycan 受体实现的。通过在同源异型区中实验性地引入曲率异质性，我们证实弯曲的组织几何形状将 Dfd 依赖性的平面张力转化为向内的力，从而驱动折叠。因此，平面张力和组织曲率之间的相互作用定量解释了时空折叠动力学。总的来说，我们的工作强调了遗传模式和组织几何形状如何为发育过程中的折叠形态发生提供了一个简单的设计原则。
