Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation-State Research Institute, Czartoryskich 8, 24-100 Puławy, Poland; Department of Natural Products Chemistry, Medical University of Lublin, Lublin 20-093, Poland.
Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation-State Research Institute, Czartoryskich 8, 24-100 Puławy, Poland.
Food Chem. 2023 Jun 30;412:135587. doi: 10.1016/j.foodchem.2023.135587. Epub 2023 Jan 27.
Ochradenus baccatus Delile (Resedaceae) is a desert plant with edible fruits native to the Middle East. Few investigators have reported antibacterial, antiparasitic and anti-cancer activities of the plant. Herein we evaluated the cytotoxic activity of O. baccatus using four cell lines and a zebrafish embryo model. Additionally, liquid chromatography coupled with mass spectroscopy was performed to characterize the extract's main constituents. The highest cytotoxicity was observed against human cervical adenocarcinoma (HeLa), with CC of 39.1 µg/mL and a selectivity index (SI) of 7.23 (p < 0.01). Metabolic analysis of the extract resulted in the annotation of 57 metabolites, including fatty acids, flavonoids, glucosinolates, nitrile glycosides, in addition to organic acids. The extract showed an abundance of hydroxylated fatty acids (16 peaks). Further, 3 nitrile glycosides have been identified for the first time in Ochradenus sp., in addition to 2 glucosinolates. These identified phytochemicals may partially explain the cytotoxic activity of the extract. We propose O. baccatus as a possible safe food source for further utilization to partially contribute to the increasing food demand specially in Saharan countries.
Ochradenus baccatus Delile（瑞香科）是一种原产于中东地区的沙漠植物，其果实可食用。少数研究人员曾报道过该植物具有抗菌、抗寄生虫和抗癌的活性。在此，我们采用四种细胞系和斑马鱼胚胎模型评估了 O. baccatus 的细胞毒性。此外，还采用液相色谱-质谱联用技术对提取物的主要成分进行了表征。该植物对人宫颈癌细胞（HeLa）的细胞毒性最高，CC50 为 39.1µg/mL，选择性指数（SI）为 7.23（p<0.01）。提取物的代谢分析导致鉴定出 57 种代谢产物，包括脂肪酸、类黄酮、硫代葡萄糖苷、腈糖苷，以及有机酸。提取物中含有丰富的羟基脂肪酸（16 个峰）。此外，首次在 Ochradenus 属中鉴定出 3 种腈糖苷和 2 种硫代葡萄糖苷。这些鉴定出的植物化学物质可能部分解释了提取物的细胞毒性。我们建议将 O. baccatus 作为一种可能的安全食品来源，进一步加以利用，以部分满足特别是撒哈拉以南国家日益增长的粮食需求。
