Saikia Queen, Hazarika Ajit, Kalita Jogen Chandra
Department of Zoology, Gauhati University, Guwahati, Assam, India.
Tyagbir Hem Baruah College, Jamugurihat, Sonitpur, Assam, India.
Reprod Toxicol. 2023 Apr;117:108341. doi: 10.1016/j.reprotox.2023.108341. Epub 2023 Feb 3.
Paroxetine (PRX), a widely prescribed antidepressant, often leads to sexual dysfunction. The available management options such as sildenafil (SDF), are associated with side effects. The present study investigates the fertility-boosting properties of isoliquiritigenin (ISL) on PRX-induced sexual dysfunction in male mice. We allocated fertile mice into six different groups (n = 5): group I- DMSO; group II- PRX; group III- co-administered PRX and SDF; group IV- ISL alone; group V- co-administered PRX and ISL (low dose); and, group VI- co-administered PRX and ISL (high dose). 14 days post treatment, animals were sacrificed, and the weights of the testis and epididymis were evaluated. Furthermore, sperm parameters, testicular and epididymal antioxidant levels, serum testosterone and nitric oxide (NO) levels, histoarchitecture of testis and epididymis, and markers of cellular toxicity were assessed. Results revealed that the PRX administration reduced organ weights, sperm count, intact acrosome, catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), serum testosterone, and NO levels, and increased sperm abnormalities and MDA levels (a biomarker for lipid peroxidation). Additionally, we observed damage in the testis and epididymis. The toxicity biomarker study revealed a higher concentration of SGOT, SGPT, and ALP enzymes in the PRX-treated group. However, the co-administration of PRX with ISL ameliorated the adverse effect of PRX on the parameters mentioned above. The PRX+ISL (high) results were almost at par with the PRX+SDF group. The group that received ISL alone showed overall improvements. In conclusion, our comprehensive panel of tests indicates that ISL could be helpful in managing sexual dysfunction.
帕罗西汀（PRX）是一种广泛使用的抗抑郁药，常导致性功能障碍。现有的治疗选择如西地那非（SDF），都伴有副作用。本研究调查了异甘草素（ISL）对PRX诱导的雄性小鼠性功能障碍的生育促进特性。我们将可育小鼠分为六个不同组（n = 5）：第一组 - 二甲亚砜；第二组 - PRX；第三组 - 联合使用PRX和SDF；第四组 - 单独使用ISL；第五组 - 联合使用PRX和低剂量ISL；第六组 - 联合使用PRX和高剂量ISL。治疗14天后，处死动物，评估睾丸和附睾的重量。此外，还评估了精子参数、睾丸和附睾的抗氧化水平、血清睾酮和一氧化氮（NO）水平、睾丸和附睾的组织结构以及细胞毒性标志物。结果显示，给予PRX会降低器官重量、精子数量、完整顶体、过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽（GSH）、血清睾酮和NO水平，并增加精子异常和丙二醛水平（脂质过氧化的生物标志物）。此外，我们观察到睾丸和附睾有损伤。毒性生物标志物研究显示，PRX治疗组中谷草转氨酶、谷丙转氨酶和碱性磷酸酶的浓度较高。然而，PRX与ISL联合使用可改善PRX对上述参数的不良影响。PRX + ISL（高剂量）组的结果几乎与PRX + SDF组相当。单独接受ISL的组总体上有所改善。总之，我们全面的测试表明，ISL可能有助于治疗性功能障碍。
