探讨注射用对慢性心力衰竭大鼠心功能的影响及作用机制。
Investigating the Effects and Mechanism of Injection on Cardiac Function in Rats with Chronic Heart Failure.
机构信息
Department of Geriatric Cardiology, Hebei General Hospital, NO. 348 Heping West Road, Shijiazhuang, 050050, China.
Department of Preventive Health, Hebei General Hospital, NO. 348 Heping West Road, Shijiazhuang, 050050, China.
出版信息
Comb Chem High Throughput Screen. 2023;26(12):2238-2246. doi: 10.2174/1386207326666230203145254.
AIM
To study the effect of injection on cardiac function and the reninangiotensin- aldosterone system (RASS) in rats with chronic heart failure.
BACKGROUND
injection, a traditional Chinese medication for relieving blood stasis and improving blood circulation, is an excellent therapeutic for treating coronary heart disease-angina pectoris. injection's major component, salidroside, protects the cardiovascular system. But there isn't much first-hand evidence about how injectable affects heart failure.
OBJECTIVES
In this study, a rat model of heart failure was established, and the effect of injection on myocardial cell morphology, cardiac function, and ventricular remodelling in rats with heart failure was investigated.
METHODS
66 SD male rats were selected; 10 were randomly selected as a blank control group, and 56 were treated intraperitoneally with doxorubicin (4 g/g). After 6 weeks, all animals had LVEF 60%. Established a heart failure model. Each group had 14 rats: model control, low-dose, mediumdose, and high-dose injection. The 2 mL/kg of injection was injected into the tail vein once a day for 2 weeks. Both the blank and control groups received normal daily saline. After 2 weeks, the echocardiographic index, RASS-related index, and serum BNP level were assessed in all rats, and myocardial tissue morphology was observed. MiRNA423-5p, miRNA499-5p, and miRNA210-3p were extracted from peripheral blood. Rhodiola rosea injection on its expression was compared to healthy control rats.
RESULTS
6 mL/kg Rhodiola Rosea injection lowered LVEDV and LVESV while increasing LVEF and LVFS. Injections of 6 mL/kg reduce plasma levels of miR-210-3p, miR-423- 5p, miRNA-499, and BNP in heart failure model rats. The 6 mL/kg injection can restore the RASS indexes of heart failure rats to the level of the normal group.
CONCLUSION
The present study offers preliminary evidence supporting the use of injection in the treatment of heart failure and offers a solid foundation for clinical off-label medication use.
目的
研究丹参多酚酸盐对慢性心力衰竭大鼠心功能及肾素血管紧张素醛固酮系统（RASS）的影响。
背景
丹参多酚酸盐是一种活血化瘀的中药制剂，是治疗冠心病心绞痛的良药。丹参多酚酸盐的主要成分之一，红景天苷，具有保护心血管系统的作用。但是，关于丹参多酚酸盐如何影响心力衰竭，目前还没有太多的第一手证据。
目的
本研究建立了大鼠心力衰竭模型，观察丹参多酚酸盐对心力衰竭大鼠心肌细胞形态、心功能及心室重构的影响。
方法
选择 66 只 SD 雄性大鼠，随机抽取 10 只为空白对照组，其余 56 只大鼠采用腹腔注射阿霉素（4 g/g）的方法建立心力衰竭模型。6 周后，所有大鼠 LVEF 均<60%，造模成功。每组 14 只大鼠：模型对照组、低剂量丹参多酚酸盐组、中剂量丹参多酚酸盐组、高剂量丹参多酚酸盐组。每天尾静脉注射 2 mL/kg 丹参多酚酸盐，连续 2 周。空白对照组和模型对照组给予生理盐水。2 周后，检测各组大鼠的超声心动图指标、RASS 相关指标和血清 BNP 水平，并观察心肌组织形态。提取各组大鼠外周血中的 miRNA423-5p、miRNA499-5p 和 miRNA210-3p，比较丹参多酚酸盐对其表达的影响。与健康对照组大鼠进行比较。
结果
6 mL/kg 丹参多酚酸盐可降低 LVEDV 和 LVESV，增加 LVEF 和 LVFS。6 mL/kg 丹参多酚酸盐可降低心力衰竭模型大鼠血浆 miR-210-3p、miRNA-423-5p、miRNA-499 和 BNP 水平。6 mL/kg 丹参多酚酸盐可使心力衰竭大鼠的 RASS 指标恢复到正常组水平。
结论
本研究为丹参多酚酸盐治疗心力衰竭提供了初步的证据支持，为临床超说明书用药提供了坚实的基础。
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