Bio-products from isolated from Ghanaian reduce burden in vector mosquitoes.

Novel ideas for control of mosquito-borne disease include the use of bacterial symbionts to reduce transmission. Bacteria belonging to the family Enterobacteriaceae isolated from mosquito midgut have shown promise in limiting intensity in the vector. However, the mechanism of interaction between bacteria and parasite remains unclear. This study aimed at screening bio-products of two bacteria candidates for their anti-Plasmodial effects on mosquito stages of P. and were isolated from field-caught s.l. Spent media from liquid cultures of these bacteria were filtered, lyophilized and dissolved in sterile phosphate buffered saline (PBS). The re-dissolved bacterial products were added to gametocytaemic blood meals and fed to mosquitoes membrane feeders. Control groups were fed on infected blood with or without lyophilized LB medium. The effect of the products on the infection prevalence and intensity of in mosquitoes was assessed by dissecting mosquito midguts and counting oocysts 10-11 days post-infection. bio-products elicited significant reduction in the number of mosquitoes infected (=4.02 x10) with and the oocyst intensity (<2 x 10) than products (>0.05 for both prevalence and intensity) compared to the control (lyophilized LB medium). These data support the use of bioproducts released by for malaria control based on transmission blocking in the vector.