Wang Yu, Chen Shuting, Zhang Jingyi, Ye Qing, Liu Yin
School of Medicine, Nankai University, Tianjin 300071, P. R. China.
Key Laboratory of Weak-Light Nonlinear Photonics, Ministry of Education, School of Physics and TEDA Applied Physics, Nankai University, Tianjin 300071, P. R. China.
ACS Omega. 2023 Jan 19;8(4):4385-4390. doi: 10.1021/acsomega.2c07859. eCollection 2023 Jan 31.
As a new type of nanomaterial, DNA nanomaterials show great potential in biomedical applications because of their high precision, high controllability, and high biocompatibility among other characteristics. Therapeutic drugs based on DNA nanomaterials have been shown to have beneficial therapeutic effects on a variety of diseases. The application of DNA nanomedicines in the treatment of diseases has become a rapidly developing area of study. However, the instability of DNA nanomaterials greatly limits their clinical application. Therefore, we designed and synthesized a stable topological DNA nanostructure: DNA Nano Trihedron (DNT). We demonstrated that DNT could enter MCF-7 cells without the transfection agent. In addition, DNT could induce dramatic changes in gene expression and produce significant inhibitory effects on MCF-7 cells. DNT after two months of storage still had an inhibitory effect on MCF-7 cells.
作为一种新型纳米材料，DNA纳米材料因其高精度、高可控性和高生物相容性等特性，在生物医学应用中显示出巨大潜力。基于DNA纳米材料的治疗药物已被证明对多种疾病具有有益的治疗效果。DNA纳米药物在疾病治疗中的应用已成为一个快速发展的研究领域。然而，DNA纳米材料的不稳定性极大地限制了它们的临床应用。因此，我们设计并合成了一种稳定的拓扑DNA纳米结构：DNA纳米三角体（DNT）。我们证明DNT无需转染剂即可进入MCF-7细胞。此外，DNT可诱导基因表达发生显著变化，并对MCF-7细胞产生显著抑制作用。储存两个月后的DNT对MCF-7细胞仍有抑制作用。
