LncRNA Induced Non-Small Cell Lung Cancer Development through Up-Regulating CDK6 by Sponge Adsorption of miRNA-204.

BACKGROUND

Non-coding RNA played one pivotal role in NSCLC in terms of pathogenesis and progression. We aimed to determine the LncRNA, which can be one new potential target for NSCLC treatment and its possible mechanisms from Jan 2017 to Aug 2020.

METHODS

Gene , which produced new cells in tumor cellular system, was knocked out. Its specific effects were tested in terms of cellular phenotype. was chosen to target for miRNA as well as downstream mRNA targeted by miRNA, which verified the combination predicted before. Specific impacts brought from miRNA on NSCLC cells were examined. At last, dynamic impacts produced through miRNA and on mRNA expression and NSCLC cellular phenotype were examined.

RESULTS

expression was up-regulated in tissues and cells from NSCLC. Knocking out gene could repress cellular survival and migration of A549 and H292 cells. miRNA-204 was repressed via targeting. miRNA-204 over-expressing repressed invasion ability of NSCLC cells and CDK6 targeted by miRNA-204. CDK6 knocking out suppressed survival and migration of NSCLC cells. The influence brought from gene knocking out could be reversed through suppressing miRNA-204, causing up-regulated CDK6 as well as expression in NSCLC tissues. miRNA-204 was negatively correlated with CDK6 as well as , respectively. Nonetheless, CDK6 possessed the positive relationship with .

CONCLUSION

An axis of lncRNA /miRNA-204/CDK6 can modulate NSCLC cells in terms of migration as well as survival.