比较圆锥角膜患者和健康受试者的维生素 B12、叶酸、核黄素和同型半胱氨酸的血液水平。

Comparison of blood levels of vitamin B12, folic acid, riboflavin, and homocysteine in keratoconus and healthy subjects.

机构信息

From the Ophthalmology Clinic, Emirdag State Hospital, Afyonkarahisar, Turkey (Sozer); Ophthalmology Clinic, Private Gürlife Hospital, Eskişehir, Turkey (Ozalp); Department of Ophthalmology, Eskişehir Osmangazi University Medical School, Eskişehir, Turkey (Atalay, Yildirim); Medical Biochemistry Clinic, Sandikli State Hospital, Afyonkarahisar, Turkey (Demir); Department of Medical Biochemistry, Eskişehir Osmangazi University Medical School, Eskişehir, Turkey (Alatas).

出版信息

J Cataract Refract Surg. 2023 Jun 1;49(6):589-594. doi: 10.1097/j.jcrs.0000000000001160.

DOI:10.1097/j.jcrs.0000000000001160

PMID:36745841

Abstract

PURPOSE

To evaluate blood levels of vitamin B12, folic acid, riboflavin, and homocysteine in keratoconus (KC) and healthy subjects.

SETTING

Eskişehir Osmangazi University, Eskişehir, Turkey.

DESIGN

Cross-sectional study.

METHODS

100 KC patients (patient group) between the ages of 18 to 35 years and 200 healthy individuals (control group) in the same age range were included in the Eskişehir Osmangazi University Hospital Eye Clinic between October 2019 and March 2020. In all cases, a complete ophthalmologic examination and corneal tomography evaluation with a Pentacam Scheimpflug camera were performed. In blood samples, vitamin B12 and folic acid levels were measured using an electrochemiluminescence immunoassay analyzer, and homocysteine and riboflavin levels were measured using high-performance liquid chromatography. Chi-square tests were used in the analysis of categorical variables, and Mann-Whitney U and Kruskal-Wallis tests were used in the analysis of numerical variables.

RESULTS

Homocysteine (13.0 ± 6.6 vs 12.1 ± 5.4 μmol/L, P = .190), vitamin B12 (313.5 ± 119.4 vs 322.9 ± 128.3 pg/mL, P = .619), and folic acid (7.0 ± 2.7 vs 7.4 ± 2.9 ng/mL, P = .230) levels were not different between KC (100 eyes of 100 subjects) and control (200 eyes of 200 subjects) groups. The mean riboflavin level was 84.0 ± 21.8 μg/L in the patient group and 183.6 ± 74.3 μg/L in the control group, with a significant difference between the 2 groups ( P < .001). Riboflavin levels were below 180 μg/L in 99% (n = 99) of the cases in the KC group and 53.5% (n = 107) in the control group ( P < .001).

CONCLUSIONS

Low blood riboflavin levels in KC patients may be a possible risk factor in the pathogenesis of KC.

摘要

目的

评估维生素 B12、叶酸、核黄素和同型半胱氨酸在圆锥角膜（KC）和健康受试者中的血液水平。

地点

土耳其埃斯基谢希尔奥斯曼加济大学。

设计

横断面研究。

方法

2019 年 10 月至 2020 年 3 月期间，在埃斯基谢希尔奥斯曼加济大学医院眼科诊所纳入 100 名年龄在 18 至 35 岁之间的 KC 患者（患者组）和 200 名年龄相同的健康个体（对照组）。所有患者均接受全面眼科检查和 Pentacam Scheimpflug 相机角膜断层扫描评估。在血液样本中，使用电化学发光免疫分析器测量维生素 B12 和叶酸水平，使用高效液相色谱法测量同型半胱氨酸和核黄素水平。采用卡方检验分析分类变量，采用 Mann-Whitney U 和 Kruskal-Wallis 检验分析数值变量。

结果

同型半胱氨酸（13.0±6.6 比 12.1±5.4 μmol/L，P=.190）、维生素 B12（313.5±119.4 比 322.9±128.3 pg/mL，P=.619）和叶酸（7.0±2.7 比 7.4±2.9 ng/mL，P=.230）水平在 KC（100 只眼，100 例）和对照组（200 只眼，200 例）之间无差异。患者组平均核黄素水平为 84.0±21.8 μg/L，对照组为 183.6±74.3 μg/L，两组间差异有统计学意义（P <.001）。KC 组 99%（n=99）的病例核黄素水平低于 180 μg/L，对照组为 53.5%（n=107）（P <.001）。