Su Yue, Cheng Shasha, Ding Yixin, Wang Linge, Sun Mingshuang, Man Chaoxin, Zhang Yu, Jiang Yujun
Key Laboratory of Dairy Science, Ministry of Education, Department of Food Science, Northeast Agricultural University, Harbin 150030, China.
Key Laboratory of Dairy Science, Ministry of Education, Department of Food Science, Northeast Agricultural University, Harbin 150030, China.
Int J Biol Macromol. 2023 Apr 1;233:123558. doi: 10.1016/j.ijbiomac.2023.123558. Epub 2023 Feb 4.
The intestinal barrier protects the host from harmful substances. This paper investigated two polysaccharides extracted from the Hericium erinaceus before and after fermentation (HEP and FHEP). The effects of two polysaccharides on the intestinal barrier were investigated in cell and mice models. The results showed that polysaccharides had a protective effect against acrylamide-induced injury in IEC-6 cell. Compared with HEP, FHEP significantly increased TEER and paracellular permeability (P < 0.05). Both polysaccharides the expression of alter tight junction (TJ) and mucin (MUC) as observed in cell Western Bolt (WB). Polysaccharides also enhance the intestinal barrier function in mice by improving cyclophosphamide induced cytokines level, TJ and MUC expression, and gut microbiota. The results showed that FHEP significantly increased IgA, IgG, and IgM levels while decreasing TNF-, IL-1, and IL-6 levels (P < 0.05). The immunohistochemical results showed that both polysaccharides significantly increased the expression of occludin, ZO-1, ZO-2, claudin-3, claudin-4, MUC2 and decreased claudin-2. In parallel, polysaccharides could alter the composition of the gut microbiota, indicating that increased in Bacteriodetes, Firmicutes and decreased in Klebsiella and Shigella. This work provides important views on the protective effect of fermented polysaccharides on the intestinal barrier, and provides a potential mechanism for the beneficial health properties of these biomacromolecules.
肠道屏障可保护宿主免受有害物质侵害。本文研究了从发酵前后的猴头菌中提取的两种多糖（HEP和FHEP）。在细胞和小鼠模型中研究了这两种多糖对肠道屏障的影响。结果表明，多糖对丙烯酰胺诱导的IEC-6细胞损伤具有保护作用。与HEP相比，FHEP显著提高了跨上皮电阻（TEER）和细胞旁通透性（P<0.05）。在细胞蛋白质免疫印迹（WB）分析中观察到，两种多糖均改变了紧密连接（TJ）和黏蛋白（MUC）的表达。多糖还通过改善环磷酰胺诱导的细胞因子水平、TJ和MUC表达以及肠道微生物群来增强小鼠的肠道屏障功能。结果表明，FHEP显著提高了IgA、IgG和IgM水平，同时降低了TNF-、IL-1和IL-6水平（P<0.05）。免疫组化结果表明，两种多糖均显著增加了闭合蛋白、紧密连接蛋白1（ZO-1）、紧密连接蛋白2（ZO-2）、claudin-3、claudin-4、MUC2的表达，并降低了claudin-2的表达。同时，多糖可以改变肠道微生物群的组成，表明拟杆菌门、厚壁菌门增加，克雷伯菌属和志贺菌属减少。这项工作为发酵多糖对肠道屏障的保护作用提供了重要见解，并为这些生物大分子的有益健康特性提供了潜在机制。
