Tian Baoming, Wang Peiyi, Xu Tianrui, Cai Ming, Mao Rongliang, Huang Liangshui, Sun Peilong, Yang Kai
College of Food Science and Technology, Zhejiang University of Technology, Huzhou 313299, China.
Changshan Haofeng Agricultural Development Co. Ltd, Quzhou 324207, China.
Food Funct. 2023 Mar 20;14(6):2921-2932. doi: 10.1039/d2fo03769f.
is a kind of large fungus with rich nutrition and its polysaccharides exhibit various biological activities. In recent years, widespread interest has been focused on maintaining or improving intestinal health through the consumption of edible fungi. Studies have shown that hypoimmunity can damage the intestinal barrier, which in turn seriously affects human health. The aim of this work was to investigate the ameliorative effects of polysaccharides (HEPs) on intestinal barrier damage in cyclophosphamide (CTX)-induced immunocompromised mice. The results showed that the HEP effectively increased the levels of total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-PX), and total superoxide dismutase (T-SOD), and decreased malondialdehyde (MDA) content in the liver tissues of mice. In addition, the HEP restored the immune organ index, increased the serum levels of IL-2 and IgA, augmented the mRNA expression levels of intestinal Muc2, Reg3γ, occludin and ZO-1, and reduced intestinal permeability in mice. It was further confirmed by an immunofluorescence assay that the HEP enhanced the expression level of intestinal tight junction proteins to protect the intestinal mucosal barrier. These results suggested that the HEP could reduce intestinal permeability and enhance intestinal immune functions by increasing antioxidant capacity, tight junction proteins and immune-related factors in CTX-induced mice. In conclusion, the HEP effectively ameliorated CTX-induced intestinal barrier damage in immunocompromised mice, which provides a new application direction for the HEP as a natural immunopotentiator with antioxidant function.
是一种营养丰富的大型真菌，其多糖具有多种生物活性。近年来，通过食用食用菌来维持或改善肠道健康受到广泛关注。研究表明，免疫力低下会损害肠道屏障，进而严重影响人类健康。这项工作的目的是研究多糖（HEPs）对环磷酰胺（CTX）诱导的免疫功能低下小鼠肠道屏障损伤的改善作用。结果表明，HEP能有效提高小鼠肝脏组织中总抗氧化能力（T-AOC）、谷胱甘肽过氧化物酶（GSH-PX）和总超氧化物歧化酶（T-SOD）的水平，并降低丙二醛（MDA）含量。此外，HEP恢复了免疫器官指数，提高了血清IL-2和IgA水平，增强了肠道Muc2、Reg3γ、闭合蛋白和ZO-1的mRNA表达水平，并降低了小鼠的肠道通透性。免疫荧光测定进一步证实，HEP增强了肠道紧密连接蛋白的表达水平，以保护肠道黏膜屏障。这些结果表明，HEP可以通过提高CTX诱导小鼠的抗氧化能力、紧密连接蛋白和免疫相关因子来降低肠道通透性并增强肠道免疫功能。总之，HEP有效改善了CTX诱导的免疫功能低下小鼠的肠道屏障损伤，为HEP作为具有抗氧化功能的天然免疫增强剂提供了新的应用方向。
