Ratanachamnong Piyanee, Chunchaowarit Yotsayut, Namchaiw Poommaree, Niwaspragrit Cholticha, Rattanacheeworn Punyabhorn, Jaisin Yamaratee
Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Department of Pharmacology, Faculty of Medicine, Srinakharinwirot University, Bangkok, Thailand.
Heliyon. 2023 Jan 24;9(2):e13068. doi: 10.1016/j.heliyon.2023.e13068. eCollection 2023 Feb.
or kaffir lime is a native tropical plant containing a high level of phenolic and flavonoid compounds. Its fruits are used as a food ingredient to enhance the sour-sweet scent and flavor in many dishes. Due to its polyphenol-containing, it has also been used as traditional medicine for health benefits including oral and gum health, stress relief, hair care, and skincare. In this study, we demonstrated the antioxidant activity of water extract and its effect on human keratinocyte and fibroblast migration. The extract showed a high amount of phenolic and flavonoid contents. The HPLC analysis indicated the presence of gallic acid, catechin, caffeic acid, rutin, and quercetin. We showed that water extract exhibited free radical scavenging capacity, determined by DPPH assay, with IC of 14.91 mg/mL, and nitrite radical scavenging capacity, determined by NO assay, with IC of 4.46 mg/mL. The water extract displayed unnoticeable toxicity at all tested doses. We showed that the treatment of water extracts as low as 50 μg/mL decreased the reactive oxygen species (ROS) from HO-induced ROS formation in both cell lines. Besides, water extract promoted cell migration in a dose-dependent manner. Together, these results demonstrated the positive benefit of water extract as a wound-healing accelerator. Its health benefits may be due to the antioxidant capability of its phytochemical compounds contained in water extract that enhances the migration of two major cell types: fibroblast and keratinocytes, responsible for the proliferation and remodeling phase of wound healing.
卡菲尔酸橙是一种本地热带植物，含有高水平的酚类和黄酮类化合物。其果实被用作食品配料，以增强许多菜肴中的酸甜气味和风味。由于其含有多酚，它也被用作传统药物，对健康有益，包括口腔和牙龈健康、缓解压力、头发护理和皮肤护理。在本研究中，我们证明了水提取物的抗氧化活性及其对人角质形成细胞和成纤维细胞迁移的影响。该提取物显示出大量的酚类和黄酮类成分。高效液相色谱分析表明存在没食子酸、儿茶素、咖啡酸、芦丁和槲皮素。我们表明，通过DPPH法测定，水提取物具有自由基清除能力，IC50为14.91mg/mL，通过NO法测定，具有亚硝酸盐自由基清除能力，IC50为4.46mg/mL。在所有测试剂量下，水提取物均未显示出明显的毒性。我们表明，低至50μg/mL的水提取物处理可降低两种细胞系中由H2O2诱导的ROS形成所产生的活性氧（ROS）。此外，水提取物以剂量依赖的方式促进细胞迁移。总之，这些结果证明了水提取物作为伤口愈合促进剂的积极益处。其健康益处可能归因于水提取物中所含植物化学化合物的抗氧化能力，该能力增强了两种主要细胞类型（成纤维细胞和角质形成细胞）的迁移，这两种细胞负责伤口愈合的增殖和重塑阶段。
