Proença Diogo N, Whitman William B, Shapiro Nicole, Woyke Tanja, Kyrpides Nikos C, Morais Paula V
University of Coimbra, Centre for Mechanical Engineering, Materials and Processes, Department of Life Sciences, Calçada Martim de Freitas, 3000-456 Coimbra, Portugal.
Department of Microbiology, 527 Biological Sciences Building, University of Georgia, Athens, GA 30602-2605, USA.
Int J Syst Evol Microbiol. 2022 Dec;72(12). doi: 10.1099/ijsem.0.005623.
Bacterial strain A52C2 was isolated from the endophytic microbial community of a tree trunk and characterized. Strain A52C2 stained Gram-negative and formed rod-shaped cells that grew optimally at 30 °C and at pH 6.0-7.0. The G+C content of the DNA was 65.1 mol %. The respiratory quinone was ubiquinone 10, and the major fatty acids were cyclo-C 8 and C, representing 70.1 % of the total fatty acids. Phylogenetic analyses based on the 16S rRNA gene sequences placed strain A52C2 in a distinct lineage within the order , family . The 16S rRNA gene sequence similarities of A52C2 to that of and were 93.15 and 93.2 %, respectively. The draft genome sequence of strain A52C2 comprises 4 196 045 bases with a 195-fold mapped coverage of the genome. The assembled genome consists of 43 contigs of more than 1 000 bp ( contig size was 209 720 bp). The genome encodes 4033 putative coding sequences. The phylogenetic, phenotypic and chemotaxonomic data showed that strain A52C2 (=UCCCB 130=CECT 8949=LMG 29042) represents the type of a novel species and genus, for which we propose the name gen. nov., sp. nov.
细菌菌株A52C2是从树干的内生微生物群落中分离出来并进行了特征描述。菌株A52C2革兰氏染色阴性，形成杆状细胞，在30°C和pH 6.0 - 7.0条件下生长最佳。DNA的G + C含量为65.1 mol%。呼吸醌为泛醌10，主要脂肪酸为环C8和C，占总脂肪酸的70.1%。基于16S rRNA基因序列的系统发育分析将菌株A52C2置于目、科内的一个独特谱系中。A52C2与和的16S rRNA基因序列相似性分别为93.15%和93.2%。菌株A52C2的基因组草图序列包含4196045个碱基，基因组的映射覆盖率为195倍。组装后的基因组由43个超过1000 bp的重叠群组成（最大重叠群大小为209720 bp）。该基因组编码4033个推定的编码序列。系统发育、表型和化学分类学数据表明，菌株A52C2（=UCCCB 130=CECT 8949=LMG 29042）代表一个新物种和新属的类型，我们为此提议命名为新属，新种。
