Department of Life Sciences and CEMMPRE, FCTUC, University of Coimbra, 3000-456 Coimbra, Portugal.
Department of Microbiology, 527 Biological Sciences Building, University of Georgia, Athens, GA 30602-2605, USA.
Syst Appl Microbiol. 2018 Mar;41(2):94-100. doi: 10.1016/j.syapm.2017.11.006. Epub 2017 Dec 22.
Bacterial strain B29T1 was isolated from the endophytic microbial community of a Pinus pinaster tree trunk and characterized. Strain B29T1 stained Gram-negative and formed diplococci that grew optimally at 26-30°C and at pH 6.0-7.0. The G+C content of the DNA was 61.6mol%. The respiratory quinone was ubiquinone 10 (UK-10), and the major fatty acids were C, cyclo-Cω8c and C 12-methyl, representing 64% of the total fatty acids. Phylogenetic analyses based on the 16S rRNA gene sequences placed strain B29T1 within the order Rhodospirillales in a distinct lineage that also included the genus Geminicoccus. The 16S rRNA gene sequence similarities of B29T1 to that of Candidatus Alysiomicrobium bavaricum, Geminicoccus roseus and Candidatus Alysiosphaera europeae were 92.6%, 89.9% and 89.2%, respectively. The analysis of the available genomes from the closest families showed 177 core genes that reveals a novel family-level clade including the type strain of the genus Geminicoccus and the strain B29T1. Analysis of B29T1 genome revealed all the genes involved in autotrophic carbon dioxide fixation via the reductive pentose phosphate pathway and genes encoding for starch/glycogen and chitin degradation. The phylogenetic, phenotypic and chemotaxonomic data showed that strain B29T1 (=CIP 110763, =LMG 27745) represents the type of a novel species and genus, for which we propose the name Arboriscoccus pini gen. nov., sp. nov. A new family (Geminicoccaceae fam. nov.) is proposed for Arboriscoccus, Geminicoccus, Candidatus Alysiomicrobium and Candidatus Alysiosphaera. The description of the Geminicoccus roseus DSM 18922 is also emended.
细菌菌株 B29T1 从油松树干内生微生物群落中分离得到，并进行了表征。菌株 B29T1 革兰氏染色阴性，形成双球菌，最佳生长温度为 26-30°C，最佳 pH 值为 6.0-7.0。DNA 的 G+C 含量为 61.6mol%。呼吸醌为泛醌 10（UK-10），主要脂肪酸为 C、环-Cω8c 和 C 12-甲基，占总脂肪酸的 64%。基于 16S rRNA 基因序列的系统发育分析将菌株 B29T1 置于红螺旋菌目中的一个独特谱系内，该谱系还包括 Geminicoccus 属。B29T1 与 Candidatus Alysiomicrobium bavaricum、Geminicoccus roseus 和 Candidatus Alysiosphaera europeae 的 16S rRNA 基因序列相似性分别为 92.6%、89.9%和 89.2%。对最接近的家族的现有基因组进行分析显示，有 177 个核心基因，揭示了一个包括 Geminicoccus 属的模式菌株和菌株 B29T1 的新型家族水平分支。B29T1 基因组分析显示，所有参与通过还原性戊糖磷酸途径固定自养二氧化碳的基因和编码淀粉/糖原和几丁质降解的基因都存在。系统发育、表型和化学分类学数据表明，菌株 B29T1（=CIP 110763，=LMG 27745）代表了一个新种和新属的模式菌株，我们建议将其命名为 Arboriscoccus pini gen. nov.，sp. nov.。为 Arboriscoccus、Geminicoccus、Candidatus Alysiomicrobium 和 Candidatus Alysiosphaera 提出了一个新的科（Geminicoccaceae fam. nov.）。还对 Geminicoccus roseus DSM 18922 的描述进行了修正。
