Kim Young-Ok, Park In-Suk, Kim Dong-Gyun, Park Sooyeon, Han Sung Hoon, Yoon Jung-Hoon
Biotechnology Research Division, National Institute of Fisheries Science (NIFS), Gijang, Busan 46083, Republic of Korea.
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon 16419, Republic of Korea.
Int J Syst Evol Microbiol. 2022 Dec;72(12). doi: 10.1099/ijsem.0.005593.
A Gram-stain-negative, facultatively anaerobic, flagellated and coccoid, ovoid or rod-shaped bacterial strain, NIFS-20-8, was isolated from the intestine of an olive flounder () from the East Sea, Republic of Korea. The neighbour-joining phylogenetic tree of 16S rRNA gene sequences showed that strain NIFS-20-8 fell within the clade comprising the type strains of species. Strain NIFS-20-8 exhibited 16S rRNA gene sequence similarities of 97.2 and 97.1 % to the type strains of and , respectively, and of 96.6-97.0 % to the type strains of the other species. The average nucleotide identity and digital DNA-DNA hybridization values between the genomic sequence of strain NIFS-20-8 and those of the type strains of four species were 73.8-75.0 and 19.8-21.1 %, respectively. The DNA G+C content of strain NIFS-20-8 from genomic sequence data was 50.55 mol%. Strain NIFS-20-8 contained Q-8 as the predominant ubiquinone and summed feature 3 (C 7 and/or C 6), C and C 7 as the major fatty acids. The major polar lipids detected in stain NIFS-20-8 were phosphatidylethanolamine and phosphatidylglycerol. Distinguishing phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain NIFS-20-8 is separated from recognized species. On the basis of the data presented here, strain NIFS-20-8 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NIFS-20-8 (= KCTC 82873=NBRC 115237).
