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sp. nov.,分离自海水。

sp. nov., isolated from seawater.

机构信息

Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, South Korea.

出版信息

Int J Syst Evol Microbiol. 2019 Oct;69(10):3062-3067. doi: 10.1099/ijsem.0.003590.

Abstract

A Gram-stain-negative, aerobic, motile and rod-shaped bacterial strain, designated OISW-25, was isolated from seawater in Republic of Korea. Strain OISW-25 grew optimally at 25 °C and in the presence of 2.0 % (w/v) NaCl. The phylogenetic trees based on 16S rRNA gene sequences showed that strain OISW-25 fell within the clade comprising the type strains of species. Strain OISW-25 exhibited 16S rRNA gene sequence similarity values of 97.5, 97.2 and 97.1 % to the type strains of , and , respectively, and of 93.6-96.6 % to the type strains of the other species. The average nucleotide identity values between strain OISW-25 and ATCC BAA-637 and two non-type strains of were 78.16-79.35 % and DNA-DNA relatedness value of strain OISW-25 with the type strain of . was 17 %. The DNA G+C content of strain OISW-25 was 39.2 mol% (HPLC) or 38.7 mol% (genome data). Strain OISW-25 contained Q-8 as the predominant ubiquinone and summed feature 3 (C 7 and/or C 6) and C as the major fatty acids. The major polar lipids of strain OISW-25 were phosphatidylethanolamine and phosphatidylglycerol. Distinguished phenotypic properties, along with the phylogenetic and genetic distinctiveness, revealed that strain OISW-25 is distinct from species. On the basis of the data presented, strain OISW-25 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is OISW-25 (=KCTC 62426=NBRC 113187).

摘要

一株革兰氏阴性、需氧、能运动的杆状细菌菌株,被命名为 OISW-25,从韩国海域的海水中分离得到。菌株 OISW-25 在 25°C 和 2.0%(w/v)NaCl 的条件下生长最佳。基于 16S rRNA 基因序列的系统发育树表明,菌株 OISW-25 属于 种的模式菌株所在的分支。菌株 OISW-25 与 种的模式菌株的 16S rRNA 基因序列相似性值分别为 97.5%、97.2%和 97.1%,与其他 种的模式菌株的相似性值为 93.6%-96.6%。菌株 OISW-25 与 ATCC BAA-637 和 两种非模式 种的平均核苷酸同一性值为 78.16-79.35%,与 种的模式菌株的 DNA-DNA 相关性值为 17%。菌株 OISW-25 的 DNA G+C 含量为 39.2mol%(HPLC)或 38.7mol%(基因组数据)。菌株 OISW-25 含有 Q-8 作为主要的泛醌,总和特征 3(C 7 和/或 C 6)和 C 作为主要的脂肪酸。菌株 OISW-25 的主要极性脂为磷脂酰乙醇胺和磷脂酰甘油。显著的表型特征,以及系统发育和遗传上的独特性,表明菌株 OISW-25 与 种不同。基于所提出的数据,菌株 OISW-25 被认为代表了属的一个新种,提议将其命名为 sp. nov.。模式菌株为 OISW-25(=KCTC 62426=NBRC 113187)。

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