Li Jiayi, Liu Lirui, Huang Yuhan, Pan Jie, Li Meng
Archaeal Biology Center, Institute for Advanced Study, Shenzhen University, Shenzhen 518057, PR China.
Shenzhen Key Laboratory of Marine Microbiome Engineering, Institute for Advanced Study, Shenzhen University, Shenzhen 518057, PR China.
Int J Syst Evol Microbiol. 2023 Feb;73(1). doi: 10.1099/ijsem.0.005673.
A Gram-stain-negative, strictly aerobic, motile, rod-shaped bacterium, FT117, was isolated from mangrove sediment collected in Shenzhen, Guangdong, PR China. Growth occurred at 20-45 °C (optimum, 40 °C), pH 6-10 (optimum, 8) and in the presence of 0-5 % NaCl (optimum, 0 %). The results of 16S rRNA gene sequence analysis indicated that the identity between FT117 and CC-CFT501 was the highest (98.7 %), followed by JCM 31785 (93.6 %), CCUG 34794 (93.6 %) and LMG 31012 (93.5 %). The main fatty acids (>10 %) were C (35.8 %), cyclo-C (18.5 %) and summed feature 3 (18.1 %). The polar lipids contained phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids, two unidentified aminophospholipids and one unidentified lipid. The sole respiratory quinone was ubiquinone-8 (Q-8). Its DNA G+C content was 70.6 % (data from the genome sequence) and the estimated genome size was 3.860 Mb. The average nucleotide identity values between the FT117 genome and the genomes of CC-CFT501, JCM 31785, CCUG 34794 and LMG 31012 were 85.4 %, 76.4 %, 73.0 % and 71.3 %, and the digital DNA-DNA hybridization values were 29.1 %, 21.0 %, 20.3 % and 19.1 %, respectively. The phylogenetic, phenotypic and chemotaxonomic differences between FT117 and its phylogenetic relatives indicate that FT117 should be regarded as representing a novel species within the genus , for which the name sp. nov. is proposed. The type strain is FT117 (=KCTC 92314 = MCCC 1K07396).
从中国广东省深圳市采集的红树林沉积物中分离出一株革兰氏阴性、严格需氧、具运动性的杆状细菌FT117。该菌在20 - 45 °C（最适温度为40 °C）、pH 6 - 10（最适pH 8）以及0 - 5 % NaCl（最适盐浓度为0 %）条件下生长。16S rRNA基因序列分析结果表明，FT117与CC - CFT501的相似度最高（98.7 %），其次是JCM 31785（93.6 %）、CCUG 34794（93.6 %）和LMG 31012（93.5 %）。主要脂肪酸（>10 %）为C（35.8 %）、环丙烷脂肪酸（18.5 %）和特征性脂肪酸3（18.1 %）。极性脂质包含磷脂酰乙醇胺、双磷脂酰甘油、磷脂酰甘油、两种未鉴定的磷脂、两种未鉴定的氨基磷脂和一种未鉴定的脂质。唯一的呼吸醌为泛醌 - 8（Q - 8）。其DNA G + C含量为70.6 %（来自基因组序列数据），估计基因组大小为3.860 Mb。FT117基因组与CC - CFT501、JCM 31785、CCUG 34794和LMG 31012基因组之间的平均核苷酸一致性值分别为85.4 %、76.4 %、73.0 %和71.3 %，数字DNA - DNA杂交值分别为29.1 %、21.0 %、20.3 %和19.1 %。FT117与其系统发育亲缘关系菌株之间的系统发育、表型和化学分类学差异表明，FT117应被视为代表该属内的一个新物种，为此提出新物种名称为sp. nov.。模式菌株为FT117（=KCTC 92314 = MCCC 1K07396）。
