Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, 145, XingDa Rd, Taichung, 40227, Taiwan.
Innovation and Development Center of Sustainable Agriculture, National Chung Hsing University, Taichung, Taiwan.
Antonie Van Leeuwenhoek. 2021 Dec;114(12):2101-2111. doi: 10.1007/s10482-021-01664-x. Epub 2021 Oct 2.
A polyphasic taxonomic approach was used to characterize a Gram-stain-negative bacterium, designated strain CC-CFT501, harboring xenobiotic- and allelochemical-metabolizing genes, isolated from a long-term ecological research field in Taiwan. Cells of strain CC-CFT501 were catalase- and oxidase-positive, non-motile and short rods. Optimal growth occurred at 30 °C, pH 8 and 1% NaCl. Strain CC-CFT501 was found to share high 16S rRNA gene sequence similarity with the members of genera Quisquiliibacterium (94.3%, n = 1), Pandoraea (93.4-94.0%, n = 23) and Paraburkholderia (93.3-94.0%, n = 9), affiliated to the family Burkholderiaceae. Strain CC-CFT501 shared 76.4% orthologous average nucleotide identity (OrthoANI) and 20.9% digital DNA-DNA hybridization (dDDH) values with Quisquiliibacterium transsilvanicum DSM 29781. Draft genome sequence (3.83 Mb) of strain CC-CFT501 revealed several genes encoding the proteins involved in biphenyl and phenolic acid metabolism. Fatty acid profile contained C, C, C 3-OH, C ω7c/C ω6c and C ω7c/C ω6c in predominant amounts. The polar lipid profile consisted of phosphatidylethanolamine, thirteen unidentified amino lipids, two unidentified phospholipids and two unidentified glycolipids. The major polyamine was spermidine and ubiquinone Q-8 was the sole respiratory quinone. The DNA G + C content was 70.0 mol%. Based on its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence, ANI and dDDH analyses, strain CC-CFT501 is considered to represent a novel genus and species of the family Burkholderiaceae, for which the name Zeimonas arvi gen. nov., sp. nov. is proposed. The type strain of the type species is CC-CFT501 (= BCRC 81218 = JCM 33506).
采用多相分类法对一株革兰氏阴性细菌进行了表征，该细菌命名为 CC-CFT501 菌株，其具有代谢外来化合物和化感物质的基因，分离自台湾一个长期生态研究领域。CC-CFT501 菌株的细胞呈过氧化氢酶和氧化酶阳性，不运动，呈短杆状。最佳生长条件为 30°C、pH8 和 1%NaCl。CC-CFT501 菌株与 Quisquiliibacterium（94.3%，n=1）、Pandoraea（93.4-94.0%，n=23）和 Paraburkholderia（93.3-94.0%，n=9）属的成员具有高的 16S rRNA 基因序列相似性，属于伯克霍尔德氏菌科。CC-CFT501 菌株与 Quisquiliibacterium transsilvanicum DSM 29781 的同源平均核苷酸同一性（OrthoANI）为 76.4%，数字 DNA-DNA 杂交（dDDH）值为 20.9%。CC-CFT501 菌株的基因组草图（3.83 Mb）显示了一些编码参与联苯和酚酸代谢的蛋白质的基因。脂肪酸组成主要含有 C、C、C 3-OH、C ω7c/C ω6c 和 C ω7c/C ω6c。极性脂谱由磷脂酰乙醇胺、十三种未鉴定的氨基酸脂、两种未鉴定的磷脂和两种未鉴定的糖脂组成。主要多胺是亚精胺，唯一的呼吸醌是泛醌 Q-8。DNA G+C 含量为 70.0 mol%。基于其独特的系统发育、表型和化学分类特征以及比较 16S rRNA 基因序列、ANI 和 dDDH 分析的结果，CC-CFT501 菌株被认为代表伯克霍尔德氏菌科的一个新属和新种，因此提议建立一个新属 Zeimonas arvi 属，新种 Zeimonas arvi 种。模式种的模式菌株为 CC-CFT501（=BCRC 81218=JCM 33506）。
