Kim Juseok, Song Hye Seon, Kim Joon Yong, Whon Tae Woong, Chung Won-Hyong, Nam Young-Do, Choi Yoon-E, Roh Seong Woon
Microbiology and Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea.
Division of Environmental Science & Ecological Engineering, Korea University, Seoul 02841, Republic of Korea.
Int J Syst Evol Microbiol. 2023 Feb;73(1). doi: 10.1099/ijsem.0.005666.
A novel bacterial strain, CBA7301, was isolated from human faeces and was characterised using a polyphasic taxonomic approach. A phylogenetic analysis based on 16S rRNA gene sequences revealed that CBA7301 represented a member of the genus , in the family . The similarity between the 16S rRNA gene sequence of CBA7301 and that of its most closely related species, JCM 17102, was 96.2 %, and the average nucleotide identity between these two strains was 77.9 %. The genome size was 6 782 182 bp, and the DNA G+C content was 42.5 mol%. Cells of CBA7301 were Gram-stain-negative, strictly anaerobic and rod-shaped. The optimal growth of this organism occurred at 30-35 °C, pH 7.0 and 0.5 % (w/v) NaCl. The respiratory quinone was menaquinone 10. The predominant polar lipids were phosphatidylethanolamine, phospholipids and aminophospholipids. The major cellular fatty acid was anteiso-C. According to the results of the polyphasic taxonomic analysis, CBA7301 represents a novel species of the genus , which we named sp. nov. The type strain is CBA7301 (=KCCM 43355=ATCC TSD-227).
从人类粪便中分离出一种新型细菌菌株CBA7301，并采用多相分类法对其进行了表征。基于16S rRNA基因序列的系统发育分析表明，CBA7301代表了 科 属的一个成员。CBA7301的16S rRNA基因序列与其最密切相关的物种JCM 17102的序列相似性为96.2%，这两个菌株之间的平均核苷酸同一性为77.9%。基因组大小为6782182 bp，DNA G+C含量为42.5 mol%。CBA7301细胞革兰氏染色阴性，严格厌氧，呈杆状。该生物体的最佳生长温度为30-35°C，pH值为7.0，NaCl浓度为0.5%（w/v）。呼吸醌为甲基萘醌10。主要极性脂质为磷脂酰乙醇胺、磷脂和氨基磷脂。主要细胞脂肪酸为anteiso-C。根据多相分类分析结果，CBA7301代表 属的一个新物种，我们将其命名为 新种。模式菌株为CBA7301（=KCCM 43355=ATCC TSD-227）。
