State Key Laboratory of Microbial Biotechnology, Shandong University, Qingdao 266000, PR China.
State Key Laboratory of Microbial Resources, and Environmental Microbiology Research Center (EMRC), Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Int J Syst Evol Microbiol. 2022 May;72(5). doi: 10.1099/ijsem.0.005397.
An anaerobic bacterial strain, designated as NSJ-90, was isolated from the faeces of a healthy adult in China. Cells of strain NSJ-90 were Gram-stain-negative, non-motile, non-spore-forming and rod-shaped. Based on 16S rRNA gene sequence analysis, strain NSJ-90 belonged to the genus and was phylogenetically closely related to YIT 12056 (16S rRNA gene identity was 97.04 %). The DNA G+C content of strain NSJ-90 was 44.85 mol% (calculated from the genome). The average nucleotide identity between strain NSJ-90 and YIT 12056 was 87.60 %. The major cellular fatty acids (>10 %) of strain NSJ-90 were iso-C, anteiso-C and iso-C 3-OH. Menaquinone-10 was detected as the respiratory quinone. The major products of glucose fermentation were acetic, propionic and isovaleric acids. Based on its phylogenetic, phenotypic and chemotaxonomic characteristics, we propose that strain NSJ-90 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NSJ-90 (=CGMCC 1.17886=KCTC 25305).
从中国一名健康成年人的粪便中分离到一株厌氧细菌，命名为 NSJ-90 株。NSJ-90 株的细胞呈革兰氏阴性、不运动、不产芽孢、呈杆状。基于 16S rRNA 基因序列分析，NSJ-90 株属于 属，与 YIT 12056（16S rRNA 基因序列相似度为 97.04%）亲缘关系最近。NSJ-90 株的 DNA G+C 含量为 44.85mol%（基于基因组）。NSJ-90 株与 YIT 12056 之间的平均核苷酸同一性为 87.60%。NSJ-90 株的主要细胞脂肪酸（>10%）为 iso-C、anteiso-C 和 iso-C 3-OH。检测到menaquinone-10 作为呼吸醌。葡萄糖发酵的主要产物为乙酸、丙酸和异戊酸。基于其系统发育、表型和化学分类特征，我们建议 NSJ-90 株代表 属的一个新种，命名为 sp. nov.。其模式菌株为 NSJ-90 株（=CGMCC 1.17886=KCTC 25305）。
