Otsu Nutraceuticals Research Institute, Otsuka Pharmaceutical Co., Ltd., 3-31-13 Saigawa, Otsu, Shiga, Japan.
Int J Syst Evol Microbiol. 2023 Feb;73(1). doi: 10.1099/ijsem.0.005697.
A bacterial strain, WON2089, was isolated from the faeces of healthy Japanese adults and is able to use mucin as the sole carbon and nitrogen source. Sequencing of its 16S rRNA gene showed that WON2089 has 98.0 and 94.4% similarity to Muc and Pyt, respectively, while phylogenetic tree analysis confirmed that it belongs to the genus . The whole genome of WON2089 was sequenced, which showed that it shares 84.5 % average nucleotide identity (ANI) and 24.9 % digital DNA-DNA hybridization (dDDH) with its closest relative, Muc. Cells of WON2089 are non-motile, anaerobic and oval-shaped (0.4-0.5×0.5-1.0 µm). The strain is Gram-stain-negative and grows in the temperature range of 25-45 °C (optimum, 30-37 °C) and pH range of pH 5.5-9.5 (optimum, pH 6.5-8.0). WON2089 can utilize d-glucose, d-mannitol, lactose and d-mannose, as assessed by API20A strips. The major cellular fatty acids are C anteiso, C 3OH and C ω9 (55.5, 7.5 and 5.8 % of total fatty acids, respectively). Based on 16S rRNA sequencing, ANI, dDDH and acid formation from d-mannitol, WON2089 is distinct from previously reported species of the genus . Based on phenotypic, phylogenetic and genetic characteristics, WON2089 represents a novel species of the genus and the name sp. nov. is proposed. The type strain is WON2089 (= NBRC 115679= DSM 114407).
一株细菌菌株，WON2089，从健康日本成年人的粪便中分离出来，能够将黏蛋白作为唯一的碳源和氮源。其 16S rRNA 基因测序显示，WON2089 与 Muc 和 Pyt 的相似度分别为 98.0%和 94.4%，而系统发育树分析证实其属于 属。WON2089 的全基因组测序表明，它与最接近的亲缘种 Muc 共享 84.5%的平均核苷酸同一性 (ANI) 和 24.9%的数字 DNA-DNA 杂交 (dDDH)。WON2089 细胞是非运动的、厌氧的和椭圆形的 (0.4-0.5×0.5-1.0 μm)。该菌株革兰氏染色阴性，生长温度范围为 25-45°C (最佳温度为 30-37°C)，pH 值范围为 pH 5.5-9.5(最佳 pH 为 6.5-8.0)。WON2089 可以利用 d-葡萄糖、d-甘露醇、乳糖和 d-甘露糖，通过 API20A 条带进行评估。主要细胞脂肪酸为 C anteiso、C 3OH 和 C ω9 (分别占总脂肪酸的 55.5%、7.5%和 5.8%)。基于 16S rRNA 测序、ANI、dDDH 和 d-甘露醇酸化，WON2089 与先前报道的属种明显不同。基于表型、系统发育和遗传特征，WON2089 代表了属的一个新种，建议命名为 sp. nov.。模式菌株为 WON2089(=NBRC 115679=DSM 114407)。
