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sp. nov.,分离自海洋塑料残片。

sp. nov., isolated from marine plastic residues.

机构信息

Institute for Integrative Systems Biology I2SysBio (Universitat de València-CSIC), Calle del Catedràtic Agustin Escardino Benlloch 9, 46980 Paterna, Spain.

Darwin Bioprospecting Excellence SL. Parc Científic Universitat de València, Calle del Catedràtic Agustin Escardino Benlloch 9, 46980 Paterna, Spain.

出版信息

Int J Syst Evol Microbiol. 2023 Feb;73(1). doi: 10.1099/ijsem.0.005677.

Abstract

A novel Gram-reaction-negative, aerobic, motile, rod-shaped, grey bacterium, strain P4.10X, was isolated from plastic debris sampled from shallow waters in the Mediterranean Sea (Valencia, Spain). P4.10X was catalase- and oxidase-positive, and grew under mesophilic, neutrophilic and halophilic conditions. The 16S rRNA gene sequences revealed that P4.10X was closely related to DSM 19524 and E6 (98.25 and 98.03 % sequence similarity, respectively). The DNA G+C content of the genome sequence of P4.10X was 53.66 %. The genomic indexes average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization (dDDH) confirmed its classification as representing a novel species of the genus . The predominant fatty acids were summed feature 8 (Cω7/Cω6) and C ω 11-methyl. The results of this polyphasic study confirm that P4.10X represents a novel species of the genus , for which the name sp. nov. is proposed (type strain P4.10X=CECT 30306 = DSM 112386).

摘要

一株新型革兰氏反应阴性、需氧、能运动、杆状、灰色细菌,菌株 P4.10X,从地中海浅水区的塑料碎片中分离得到(西班牙瓦伦西亚)。P4.10X 呈过氧化氢酶和氧化酶阳性,在中温、嗜中性和嗜盐条件下生长。16S rRNA 基因序列表明,P4.10X 与 DSM 19524 和 E6 密切相关(分别为 98.25%和 98.03%的序列相似性)。P4.10X 基因组序列的 DNA G+C 含量为 53.66%。基因组指数平均核苷酸同一性(ANIb)和数字 DNA-DNA 杂交(dDDH)的数字 DNA-DNA 杂交证实,其分类代表了一个新的属种。优势脂肪酸为总和特征 8(Cω7/Cω6)和 C ω 11-甲基。该多相研究的结果证实 P4.10X 代表了一个新的属种,建议使用新种名 sp. nov.(模式菌株 P4.10X=CECT 30306=DSM 112386)。

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