Institute for Integrative Systems Biology I2SysBio (Universitat de València-CSIC), Calle del Catedràtic Agustin Escardino Benlloch 9, 46980 Paterna, Spain.
Darwin Bioprospecting Excellence SL. Parc Científic Universitat de València, Calle del Catedràtic Agustin Escardino Benlloch 9, 46980 Paterna, Spain.
Int J Syst Evol Microbiol. 2023 Feb;73(1). doi: 10.1099/ijsem.0.005677.
A novel Gram-reaction-negative, aerobic, motile, rod-shaped, grey bacterium, strain P4.10X, was isolated from plastic debris sampled from shallow waters in the Mediterranean Sea (Valencia, Spain). P4.10X was catalase- and oxidase-positive, and grew under mesophilic, neutrophilic and halophilic conditions. The 16S rRNA gene sequences revealed that P4.10X was closely related to DSM 19524 and E6 (98.25 and 98.03 % sequence similarity, respectively). The DNA G+C content of the genome sequence of P4.10X was 53.66 %. The genomic indexes average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization (dDDH) confirmed its classification as representing a novel species of the genus . The predominant fatty acids were summed feature 8 (Cω7/Cω6) and C ω 11-methyl. The results of this polyphasic study confirm that P4.10X represents a novel species of the genus , for which the name sp. nov. is proposed (type strain P4.10X=CECT 30306 = DSM 112386).
一株新型革兰氏反应阴性、需氧、能运动、杆状、灰色细菌，菌株 P4.10X，从地中海浅水区的塑料碎片中分离得到（西班牙瓦伦西亚）。P4.10X 呈过氧化氢酶和氧化酶阳性，在中温、嗜中性和嗜盐条件下生长。16S rRNA 基因序列表明，P4.10X 与 DSM 19524 和 E6 密切相关（分别为 98.25%和 98.03%的序列相似性）。P4.10X 基因组序列的 DNA G+C 含量为 53.66%。基因组指数平均核苷酸同一性（ANIb）和数字 DNA-DNA 杂交（dDDH）的数字 DNA-DNA 杂交证实，其分类代表了一个新的属种。优势脂肪酸为总和特征 8（Cω7/Cω6）和 C ω 11-甲基。该多相研究的结果证实 P4.10X 代表了一个新的属种，建议使用新种名 sp. nov.（模式菌株 P4.10X=CECT 30306=DSM 112386）。
