Institute for Integrative Systems Biology I2SysBio (Universitat de València-CSIC), Calle del Catedràtic Agustin Escardino Benlloch 9, 46980 Paterna, Spain.
Darwin Bioprospecting Excellence SL. Parc Científic Universitat de València, Calle del Catedràtic Agustin Escardino Benlloch 9, 46980 Paterna, Spain.
Int J Syst Evol Microbiol. 2021 Jul;71(7). doi: 10.1099/ijsem.0.004837.
Two novel Gram-staining-negative, aerobic, cocci-shaped, non-motile, non-spore forming, pink-pigmented bacteria designated strains T6 and T18, were isolated from a biocrust (biological soil crust) sample from the vicinity of the Tabernas Desert (Spain). Both strains were catalase-positive and oxidase-negative, and grew under mesophilic, neutrophilic and non-halophilic conditions. According to the 16S rRNA gene sequences, strains T6 and T18 showed similarities with CGMCC 1.10758 and CP2C (98.11 and 98.55% gene sequence similarity, respectively). The DNA G+C content was 69.80 and 68.96% for strains T6 and T18, respectively; the average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridization (dDDH) values confirmed their adscription to two novel species within the genus . The predominant fatty acids were summed feature 8 (Cω7Cω6), C, C 2-OH and summed feature 3 (Cω7Cω6). According to he results of the polyphasic study, strains T6 and T18 represent two novel species in the genus (which currently includes only three species), for which names sp. nov. (type strain T6 = CECT 30228=DSM 112073) and sp. nov. (type strain T18=CECT 30229=DSM 112074) are proposed, respectively.
两株新型革兰氏阴性、需氧、球形、非运动、非孢子形成、粉红色的细菌，分别命名为菌株 T6 和 T18，从西班牙塔贝纳斯沙漠（Tabernas Desert）附近的生物结皮（biological soil crust）样本中分离得到。两株菌均为过氧化氢酶阳性，氧化酶阴性，在中温、嗜中性和非嗜盐条件下生长。根据 16S rRNA 基因序列，菌株 T6 和 T18 与 CGMCC 1.10758 和 CP2C 的相似度最高（分别为 98.11%和 98.55%）。菌株 T6 和 T18 的 DNA G+C 含量分别为 69.80%和 68.96%；blast 全基因同源性（ANIb）和数字 DNA-DNA 杂交（dDDH）值的平均值证实它们属于 属内的两个新种。主要脂肪酸为 sumFeature8（Cω7Cω6）、C 16:0、C 2-OH 和 sumFeature3（Cω7Cω6）。根据多相研究结果，菌株 T6 和 T18 代表 属中的两个新种（目前该属仅包括三个种），分别命名为 sp. nov.（T6 株=CECT 30228=DSM 112073）和 sp. nov.（T18 株=CECT 30229=DSM 112074）。
