Institute for Integrative Systems Biology I2SysBio (Universitat de València-CSIC), Calle del Catedràtic Agustin Escardino Benlloch 9, 46980 Paterna, Spain.
Darwin Bioprospecting Excellence SL. Parc Científic Universitat de València, Calle del Catedràtic Agustin Escardino Benlloch 9, 46980 Paterna, Spain.
Int J Syst Evol Microbiol. 2023 Aug;73(8). doi: 10.1099/ijsem.0.005957.
A novel Gram-reaction-negative, facultatively anaerobic, rod-shaped, non-motile, non-spore forming, orange-pigmented bacterium identified as M10.2A, was isolated from marine residues submerged on the Malva-rosa beach (València, Spain), on the western coast of the Mediterranean Sea. This strain was catalase-positive and oxidase-negative and grew under mesophilic, neutrophilic and halophilic conditions. With respect to the 16S rRNA gene sequences, M10.2A showed similarities with DSM 19839 and IC154 (97.57 and 97.50 % gene sequence similarity, respectively). The genome of M10.2A was sequenced and has been deposited in the DDBJ/ENA/GenBank databases under the accession code JAKGTH000000000. The genomic DNA G+C content was 36.13 %. Its adscription to a novel species of the genus was confirmed by the genomic indexes average nucleotide identity by blast (ANIb) and digital DNA-DNA hybridisation (dDDH). The major fatty acids were iso-C, iso-CG, iso-C 3-OH, iso-C 3-OH and summed feature 3 (Cω7/Cω6). According to the results of this polyphasic study, strain M10.2A represents a novel species of the genus , for which name sp. nov. (type strain M10.2A = CECT 30308 = DSM 112385) is proposed.
一株新型革兰氏反应阴性、兼性厌氧、杆状、非运动、非孢子形成、橙色着色的细菌，被鉴定为 M10.2A，从马尔瓦-罗萨海滩（西班牙瓦伦西亚）的海洋沉积物中分离出来，位于地中海西部海岸。该菌株过氧化氢酶阳性，氧化酶阴性，在中温、嗜中性和嗜盐条件下生长。根据 16S rRNA 基因序列，M10.2A 与 DSM 19839 和 IC154 具有相似性（分别为 97.57%和 97.50%的基因序列相似性）。M10.2A 的基因组已测序，并已在 DDBJ/ENA/GenBank 数据库中以 JAKGTH000000000 登录号进行了存储。基因组 DNA G+C 含量为 36.13%。通过基因组索引平均核苷酸同一性比对（ANIb）和数字 DNA-DNA 杂交（dDDH），确认其属于 属的一个新种。主要脂肪酸为 iso-C、iso-CG、iso-C 3-OH、iso-C 3-OH 和总和特征 3（Cω7/Cω6）。根据多相研究的结果，菌株 M10.2A 代表了 属的一个新种，因此命名为 sp. nov.（模式菌株 M10.2A=CECT 30308=DSM 112385）。
