gen. nov., sp. nov., a soil actinobacterium isolated after enhanced cultivation with culture supernatant.

An actinobacterial strain, designated A5X3R13, was isolated from a compost soil suspension supplemented with extracellular material from a -culture supernatant. The strain was cultured on tenfold-diluted reasoner's 2A agar. The cells were ovoid-to-rod shaped, non-motile, Gram-stain-positive, oxidase-negative, catalase-positive and had a width of 0.5 µm and a length of 0.8-1.2 µm. The results of both 16S rRNA-based phylogenetic and whole-genome analyses indicate that A5X3R13 forms a distinct lineage within the family (order ). On the basis of the 16S rRNA gene sequence, A5X3R13 was closely related to CC-CFT486 (96.2 %), IR27-S3 (96.2 %), 130 (95.6 %), YIM 730233 (95.5 %), KSL-107 (95.4 %), 9H-4 (95.4 %), Gsoil 161 (95.3 %), and NBRC 14755 (95.2 %). The genome had a length of 4 915 757 bp, and its DNA G+C content was 68.5 mol %. The main fatty acids were 10-methyl C, C, C, C, C and iso-C. The main polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol and two unidentified phospholipids. MK-9(H) was the predominant respiratory quinone. The peptidoglycan type was A3γ (A41.1) and contained alanine, glycine, glutamic acid and ll-diaminopimelic acid in a molar ratio of 1.2 : 0.9 : 1.0 : 0.8. On the basis of the results of the phylogenetic and phenotypic analyses and comparisons with other members of the family , strain A5X3R13 is proposed to represent a novel species within a novel genus, for which the name gen. nov., sp. nov. is proposed. The type strain is A5X3R13 (=DSM 112953=NCCB 100840).