Rai Anusha, Suresh Gandham, Ria Biswas, L Vighnesh, Pk Sreya, Ipsita Sahu, Sasikala Chintalapati, Venkata Ramana Chintalapati
Department of Plant Sciences, School of Life Sciences, University of Hyderabad, P.O. Central University, Hyderabad 500 046, India.
Bacterial Discovery Laboratory, Centre for Environment, IST, JNT University Hyderabad, Kukatpally, Hyderabad-500 085, India.
Int J Syst Evol Microbiol. 2023 Feb;73(1). doi: 10.1099/ijsem.0.005672.
The members of the genus are key players in the removal of petroleum hydrocarbons from polluted marine environments. More than half of the species were described in the last decade using 16S rRNA gene phylogeny and genomic-based metrics. However, the 16S rRNA gene identity (<94 %) between some members of the genus suggested their imprecise taxonomic status. In this study, we examined the taxonomic positions of species using 16S rRNA phylogeny and further validated them using phylogenomic-related indexes such as digital DNA-DNA hybridization (dDDH), average nucleotide identity (ANI), average amino acid identity (AAI), percentage of conserved proteins (POCP) and comparative genomic studies. ANI and dDDH values confirmed that all the species were well described at the species level. The phylotaxogenomic analysis showed that species formed three clades. The inter-clade values of AAI and POCP were less than 70 %. The pan-genome evaluation depicted that the members shared 1223 core genes and its number increased drastically when analysed clade-wise. Therefore, these results necessitate the transfer of clade II and clade III members into gen. nov. and gen. nov., respectively, along with the emended description of the genus .
该属成员是从受污染海洋环境中去除石油烃的关键参与者。在过去十年中，超过一半的物种是使用16S rRNA基因系统发育和基于基因组的指标来描述的。然而，该属一些成员之间的16S rRNA基因同一性（<94%）表明它们的分类地位不精确。在本研究中，我们使用16S rRNA系统发育研究了[X]个物种的分类地位，并使用数字DNA-DNA杂交（dDDH）、平均核苷酸同一性（ANI）、平均氨基酸同一性（AAI）、保守蛋白百分比（POCP）等系统发育基因组相关指标以及比较基因组研究进一步验证了它们的分类地位。ANI和dDDH值证实所有[X]个物种在物种水平上得到了很好的描述。系统发育基因组分析表明[X]个物种形成了三个进化枝。进化枝间的AAI和POCP值小于70%。泛基因组评估显示成员共享1223个核心基因，按进化枝分析时其数量急剧增加。因此，这些结果有必要将进化枝II和进化枝III的成员分别转移到新属[属名1]和新属[属名2]中，同时对该属进行修订描述。
