Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute, No. 331 Shih-Pin Rd., Hsinchu 30062, Taiwan, ROC.
Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Int J Syst Evol Microbiol. 2022 Feb;72(2). doi: 10.1099/ijsem.0.005235.
Five Gram-stain-positive strains (M1-10, M1-13, M1-21, M2-14 and S1-1) were isolated from paper mulberry () in Taiwan. Cells were rod-shaped, non-motile, non-haemolytic, asporogenous, facultatively anaerobic, heterofermentative, and did not exhibit catalase and oxidase activities. Phylogenetic analysis of the 16S rRNA gene sequences revealed that these novel strains belonged to the genus . On the basis of 16S rRNA gene sequence similarities, the type strains of and were the closest neighbours to strains M1-10, M1-13, M1-21, M2-14 and S1-1. Sequence analyses of concatenated two partial housekeeping genes, the RNA polymerase beta subunit () and recombinase A () also indicated that the novel strains belonged to the genus . The 16S rRNA and concatenated and gene sequence similarities between strains M1-10 and M1-13 were 100 %, respectively. The average nucleotide identity values of M1-10, M1-21, M2-14 and S1-1 with and were 75.1-78.9% and 76.5-77.5 %, respectively. The digital DNA-DNA hybridization values were 19.7-21.5% and 19.6-20.4 %, respectively. Phenotypic and genotypic test results demonstrated that these strains represent four novel species of the genus , for which the names sp. nov., sp. nov., sp. nov. and sp. nov. are proposed with the type strains M1-10 (=BCRC 81237=NBRC 114433), M1-21 (=BCRC 81239=NBRC 114435), M2-14 (=BCRC 81240=NBRC 114436) and S1-1 (=BCRC 81241=NBRC 114437), respectively.
从台湾的构树中分离到五株革兰氏阳性菌株（M1-10、M1-13、M1-21、M2-14 和 S1-1）。细胞呈杆状，不运动，非溶血，无孢子，兼性厌氧，异型发酵，不表现过氧化氢酶和氧化酶活性。16S rRNA 基因序列的系统发育分析表明，这些新菌株属于 属。基于 16S rRNA 基因序列相似性， 和 的模式菌株与 M1-10、M1-13、M1-21、M2-14 和 S1-1 菌株最为接近。连接两个部分看家基因（RNA 聚合酶β亚基（）和重组酶 A（））的序列分析也表明，新菌株属于 属。M1-10 和 M1-13 菌株的 16S rRNA 和连接的 和 基因序列相似性分别为 100%。M1-10、M1-21、M2-14 和 S1-1 与 和 的平均核苷酸同一性值分别为 75.1-78.9%和 76.5-77.5%。数字 DNA-DNA 杂交值分别为 19.7-21.5%和 19.6-20.4%。表型和基因型测试结果表明，这些菌株代表 属的四个新种，分别命名为 sp. nov.、 sp. nov.、 sp. nov.和 sp. nov.，其模式菌株为 M1-10（=BCRC 81237=NBRC 114433）、M1-21（=BCRC 81239=NBRC 114435）、M2-14（=BCRC 81240=NBRC 114436）和 S1-1（=BCRC 81241=NBRC 114437）。
