Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, Jeollabuk-do 55365, Republic of Korea.
Laboratory of Microbiology, Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
Int J Syst Evol Microbiol. 2022 Nov;72(11). doi: 10.1099/ijsem.0.005614.
Strain C17-3 was isolated from blueberry fruits collected from a farmland located in Damyang-gun, Jeollanam-do, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences allocated strain C17-3 to the genus , where it occupied a rather isolated line of descent with 430A and LMG 27439 as the nearest neighbours (98.9 % sequence similarity to both species). The highest average nucleotide identity and digital DNA-DNA hybridization values were 76.3 % and 21.7 % with TBRC 12339; both values were well below the cutoff values for species delineation. Cells are strictly aerobic, Gram-stain-negative rods, catalase-positive and oxidase-negative. The DNA G+C content calculated from the genome sequence was 59.2 %. Major fatty acids were summed feature 8 (Cω6 and/or Cω7) and Ccyclo ω8. The major isoprenoid quinone was ubiquinone 9. On the basis of the results of phylogenetic analyses, phenotypic features and genomic comparisons, it is proposed that strain C17-3 represents a novel species of the genus and the name sp. nov. is proposed. The type strain is C17-3 (= KACC 21233 = LMG 31758).
菌株 C17-3 是从韩国全罗南道和顺郡一个农田中采集的蓝莓果实中分离得到的。基于 16S rRNA 基因序列的系统发育分析将菌株 C17-3 分配到属中，它与 430A 和 LMG 27439 形成了相当孤立的进化枝（与两个物种的序列相似度均为 98.9%）。与 TBRC 12339 相比，菌株 C17-3 的平均核苷酸同一性和数字 DNA-DNA 杂交值最高分别为 76.3%和 21.7%，均远低于物种划分的临界值。细胞是严格需氧的革兰氏阴性棒状，过氧化氢酶阳性，氧化酶阴性。从基因组序列计算的 DNA G+C 含量为 59.2%。主要脂肪酸为特征脂肪酸 8（Cω6 和/或 Cω7）和 Ccyclo ω8。主要的异戊二烯醌是泛醌 9。基于系统发育分析、表型特征和基因组比较的结果，建议菌株 C17-3 代表属的一个新种，并提出新种的名称。模式菌株为 C17-3（=KACC 21233=LMG 31758）。
