Department of Life Science, Chung-Ang University, Seoul 06974, Republic of Korea.
Department of Agrofood Resources, National Institute of Agricultural Sciences, RDA, Wanju-gun 55365, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Mar;70(3):2026-2033. doi: 10.1099/ijsem.0.004008.
A Gram-stain-negative, obligately aerobic bacterium, designated strain B6, was isolated from rice wine vinegar in the Republic of Korea. Cells were non-motile and oval short rods showing catalase-positive and oxidase-negative activities. Growth was observed at 15-45 °C (optimum, 30 °C) and pH 3.5-8.0 (optimum, pH 5.5-6.5). Strain B6 contained summed feature 8 (comprising Cω7 and/or C 6), and C as major fatty acids and ubiquinone-9 was identified as the sole isoprenoid quinone. The G+C content of the genomic DNA calculated from the whole genome was 53.1 mol%. Strain B6 was most closely related to LMG 1262 with very high 16S rRNA gene sequence similarity (100 %) and the strains formed a very close phylogenetic lineage together in phylogenetic trees based on 16S rRNA gene sequences. However, relatedness analyses based on concatenated amino acid sequences of 354 core genes and whole-cell MALDI-TOF profiles showed that strain B6 may form a distinct phyletic lineage from species. In addition, average nucleotide identity and DNA-DNA hybridization values between strain B6 and the type strains of species were less than 93.3 and 51.4 %, respectively. The genomic features of strain B6 were also differentiated from those of closely related type strains. Based on the phenotypic, chemotaxonomic and genomic features, strain B6 clearly represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is B6 (=KACC 21201=JCM 33371).
一株革兰氏阴性、严格需氧的细菌，被命名为 B6 菌株，从韩国米酒醋中分离得到。细胞呈非运动性的椭圆形短杆状，呈现过氧化氢酶阳性和氧化酶阴性活性。生长温度为 15-45°C（最佳温度为 30°C），pH 值为 3.5-8.0（最佳 pH 值为 5.5-6.5）。B6 菌株含有特征脂肪酸 8（包含 Cω7 和/或 C6）和 C 作为主要脂肪酸，并且鉴定出泛醌-9 是唯一的异戊二烯醌。从全基因组计算得出的基因组 DNA 的 G+C 含量为 53.1 mol%。B6 菌株与 LMG 1262 最为密切相关，16S rRNA 基因序列相似度高达 100%，并且在基于 16S rRNA 基因序列的系统发育树中，这些菌株共同形成了一个非常密切的进化分支。然而，基于 354 个核心基因的串联氨基酸序列和全细胞 MALDI-TOF 图谱的相关性分析表明，B6 菌株可能与 种形成一个独特的进化谱系。此外，B6 菌株与 种的模式菌株之间的平均核苷酸同一性和 DNA-DNA 杂交值均小于 93.3%和 51.4%。B6 菌株的基因组特征也与密切相关的 型菌株有所不同。基于表型、化学分类和基因组特征，B6 菌株清楚地代表了一个新的属种，提议将其命名为 sp. nov.。其模式菌株为 B6（=KACC 21201=JCM 33371）。
