基于基因组的分类学和功能预测，从具有促植物生长潜力的狼尾草属植物中分离到的新种福州鞘氨醇单胞菌和 Phyllosphaerae 属新种 Massilia。

Genome-based taxonomy and functional prediction of Sphingomonas fuzhouensis sp. nov. and Massilia phyllosphaerae sp. nov. isolated from Pennisetum sp. with plant growth-promoting potential.

机构信息

Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation, College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou City, Fujian Province, 350002, People's Republic of China.

Institute of Resources, Environment and Soil Fertilizer, Fujian Academy of Agricultural Sciences, Fuzhou City, Fujian Province, 35003, People's Republic of China.

出版信息

Antonie Van Leeuwenhoek. 2024 Sep 18;118(1):6. doi: 10.1007/s10482-024-02017-0.

DOI:10.1007/s10482-024-02017-0

PMID:39292388

Abstract

Two facultatively aerobic strains, designated SGZ-02 and SGZ-792, were isolated from plant Pennisetum sp., exhibiting the highest 16S rRNA gene sequence similarities with the type strains of Sphingomonas zeae LMG 28739 (98.6%) and Massilia forsythiae NBRC 114511 (98.4%), respectively. SGZ-02 grew between 5 and 45 °C, pH 5.0-11.0 and tolerated NaCl concentrations of 0-4% (w/v), whereas SGZ-792 thrived at 5-40 °C, pH 5.0-11.0 and NaCl tolerance to 0-3.5% (w/v). The major quinone of SGZ-02 was ubiquinone-10, with the dominant fatty acids being C (13.5%), Summed Feature 3 (6.3%), C2-OH (5.3%) and Summed Feature 8 (66.3%). SGZ-792 predominantly contained ubiquinone-8, with major fatty acids being C (20.3%), Summed Feature 3 (5.0%) and Summed Feature 8 (54.7%). Average nucleotide identity and digital DNA-DNA hybridization values between two strains and their closest references strains were below the bacterial species threshold. Based on genotypic and phenotypic characteristics, strains SGZ-02 and SGZ-792 are proposed as novel species within the genera Sphingomonas and Massilia, respectively. The suggested names for the new species are Sphingomonas fuzhouensis sp. nov. (SGZ-02 = GDMCC 1.4033 = JCM 36769) and Massilia phyllosphaerae sp. nov. (SGZ-792 = GDMCC 1.4211 = JCM 36643), respectively.

摘要

两株兼性需氧菌株，分别命名为 SGZ-02 和 SGZ-792，从植物 Pennisetum sp. 中分离得到，与 Sphingomonas zeae LMG 28739（98.6%）和 Massilia forsythiae NBRC 114511（98.4%）的模式菌株的 16S rRNA 基因序列相似性最高。SGZ-02 在 5-45°C、pH5.0-11.0 和耐受 0-4%（w/v）NaCl 浓度的条件下生长，而 SGZ-792 在 5-40°C、pH5.0-11.0 和耐受 0-3.5%（w/v）NaCl 的条件下生长良好。SGZ-02 的主要醌类为泛醌-10，优势脂肪酸为 C（13.5%）、Summed Feature 3（6.3%）、C2-OH（5.3%）和 Summed Feature 8（66.3%）。SGZ-792 主要含有泛醌-8，主要脂肪酸为 C（20.3%）、Summed Feature 3（5.0%）和 Summed Feature 8（54.7%）。两株菌与其最接近的参考菌株之间的平均核苷酸同一性和数字 DNA-DNA 杂交值均低于细菌种的阈值。基于基因型和表型特征，菌株 SGZ-02 和 SGZ-792 分别被提议为 Sphingomonas 和 Massilia 属中的新种。新种的建议名称分别为福州鞘氨醇单胞菌（Sphingomonas fuzhouensis）sp. nov.（SGZ-02=GDMCC 1.4033=JCM 36769）和 Phyllosphaerae 叶杆菌（Massilia phyllosphaerae）sp. nov.（SGZ-792=GDMCC 1.4211=JCM 36643）。