Liu Qiuju, Zheng Huizhen, Wang Huilin, Zhou Weicheng, Zhao Dong, Qiao Zongwei, Zheng Jia, Ren Cong, Xu Yan
Lab of Brewing Microbiology and Applied Enzymology, Key Laboratory of Industrial Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, PR China.
Wuliangye Yibin Co., Ltd., No.150 Minjiang West Road, Yibin 644007, PR China.
Int J Syst Evol Microbiol. 2022 Nov;72(11). doi: 10.1099/ijsem.0.005612.
A novel, Gram-stain-negative, rod-shaped, strictly anaerobic bacterium of genus of the phylum Bacteroidota, named strain JNU-WLY501, was isolated from pit clay used to produce strong aroma-type liquor in PR China. The genomic DNA G+C content and genome size of JNU-WLY501 were 41.4 % and 3.9 Mbp, respectively. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that JNU-WLY501 was closely related to DSM 18083 (95.7 %) and M3/6 (94.9 %). The pairwise average nucleotide identity based on blast and average amino acid identity values of JNU-WLY501 compared with M3/6 were 73.6 and 77.3 %, respectively, which both were lower than the threshold values for bacterial species delineation. The strain grew at 20-40 °C, with optimum growth at 37 °C. The pH range for growth was 5.4-9.1, with optimum growth at pH 7.5. The sodium chloride range for growth was 0.0-4.0 %, with optimum growth at 0 %. The strain did not use glucose, maltose, fructose or starch. Yeast extract, tryptone and peptone supported the growth of JNU-WLY501, and the main fermentation products were acetate and propionate. The predominant cellular fatty acids (>5 %) of JNU-WLY501 were anteiso-C (30.6 %), anteiso-C (26.1 %), C (7.7 %), iso-C (5.0 %) and iso-C (5.0 %). The respiratory quinone of JNU-WLY501 was MK-5. On the basis of the morphological, physiological, biochemical, chemotaxonomic, genotypic and phylogenetic results, JNU-WLY501 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JNU-WLY501 (=GDMCC 1.2686=JCM 34753).
从中国用于生产浓香型白酒的窖泥中分离出一种新型革兰氏阴性、杆状、严格厌氧的拟杆菌门细菌，命名为菌株JNU-WLY501。JNU-WLY501的基因组DNA G+C含量和基因组大小分别为41.4%和3.9 Mbp。基于16S rRNA基因序列的系统发育分析结果表明，JNU-WLY501与DSM 18083（95.7%）和M3/6（94.9%）密切相关。与M3/6相比，基于blast的JNU-WLY501的平均核苷酸同一性和平均氨基酸同一性值分别为73.6%和77.3%，均低于细菌物种划分的阈值。该菌株在20-40°C下生长，最适生长温度为37°C。生长的pH范围为5.4-9.1，最适生长pH为7.5。生长的氯化钠范围为0.0-4.0%，最适生长氯化钠浓度为0%。该菌株不利用葡萄糖、麦芽糖、果糖或淀粉。酵母提取物、胰蛋白胨和蛋白胨支持JNU-WLY501的生长，主要发酵产物为乙酸盐和丙酸盐。JNU-WLY501的主要细胞脂肪酸（>5%）为anteiso-C（30.6%）、anteiso-C（26.1%）、C（7.7%）、iso-C（5.0%）和iso-C（5.0%）。JNU-WLY501的呼吸醌为MK-5。基于形态学、生理学、生化、化学分类学、基因型和系统发育结果，JNU-WLY501代表该属的一个新物种，为此提出新物种名称sp. nov.。模式菌株为JNU-WLY501（=GDMCC 1.2686=JCM 34753）。
