sp. nov., isolated from bioaerosols of electronic waste.

A novel Gram-stain-negative, non-motile, spherical-shaped and facultatively anaerobic bacterial strain, designated as GB24 was isolated from bioaerosols of an E-waste dismantling site in Guiyu, Guangdong Province, South PR China. Growth occurred at 15-40 °C (optimum 37 °C), pH 5.5-9.5 (optimum 7.0), and up to 0.5 % NaCl (w/v) under aerobic conditions, GB24 was characterized taxonomically and phylogenetically. The sole isoprenoid quinone detected was ubiquinone-10 (Q-10). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, three unidentified glycolipids, one unidentified phospholipid, and one unidentified aminolipid. Carotenoid pigments were produced. The major cellular fatty acids (> 10 % of total fatty acids) were Cω6 (51.5 %) and summed feature 8 (13.5 %, comprising Cω7 and/or Cω6). Phylogenetic analysis based on 16S rRNA gene sequence and draft genome grouped strain GB24 into the genus . GB24 was most closely related to DB1506 with 97.5 % 16S rRNA gene sequence similarity. The draft genome of GB24 comprised 6 153 170 bp with a DNA G+C content of 71.5 %. The average nucleotide identity (ANI) and DNA-DNA hybridization (isDDH) values between GB24 and DB1506 were 83.2 % (Ortho ANI), 83.3 % [ANI by blast (ANIb)] and 27.0 %, respectively. Further genomic analysis of GB24 revealed the secondary metabolite clusters of terpene and phosphonate, which indicate the capacity for malleobactin (14 %) and phosphinothricin (6 %) tripeptide production. On the basis of the genotypic, chemotaxonomic and phenotypic results, GB24 represents a novel species, for which the name sp. nov. is proposed. The type strain of is GB24 (= GDMCC 1.2169 = JCM 34449).