Guo He-Bao, Zhu Hong-Tu, Zhang Yi-Fan, Yang Fu, Ma Xiao-Tong, Li Shi-Gui, Wei Hai-Lei, Zhang Xiao-Xia
State Key Laboratory of Efficient Utilization of Arable Land in China (the Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, PR China.
National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Dongying, 257300, PR China.
Curr Microbiol. 2025 Feb 7;82(3):123. doi: 10.1007/s00284-025-04090-8.
A novel Gram-stain-negative, phosphate-solubilizing and siderophore producing bacterium, which we designated as strain ACCC 02193, was separated from ore, collected in Zhongxiang, Hubei of China. Phylogenetic analysis based on 16S rRNA gene sequences showed strain ACCC 02193 is in the genus Erwinia and had highest similarities to Erwinia tasmaniensis DSM 17950 (99.32%) and Erwinia billingiae DSM 17872 (98.77%). However, phylogenomic analyses clearly showed that the strain ACCC 02193 was most closely related to Erwinia phyllosphaerae CMYE1. The strain grew at 28 °C-30 °C optimally, with 0-6% (w/v) NaCl (optimum 5%), and in the range of pH 4.0-10.0 (optimum pH 8.0). Menaquinone was identified as Q-8. The predominant fatty acids (> 10.0%) were C, C cyclo, summed feature 3 (Cω7c and/or Cω6c) and summed feature 8 (Cω7c and/or Cω6c). Genome comparisons showed that the strain shared ≤ 82.8% average nucleotide identity (ANI) and ≤ 27.2% in silico DNA-DNA hybridization (DDH) values with closely related type strains, respectively, which supported our conclusion that ACCC 02193 represented a novel species in the genus Erwinia. The DNA G+C content of the strain was 55.7%. Based on the results of physiological and biochemical characteristics, genotypic data, as well as genome comparisons, we demonstrated that type strain ACCC 02193 (= GDMCC 1.4563 = JCM 37069) is a novel species in the genus Erwinia, and we propose the name Erwinia aeris sp. nov.
从中国湖北钟祥采集的矿石中分离出一株新型革兰氏阴性、解磷和产铁载体细菌，我们将其命名为菌株ACCC 02193。基于16S rRNA基因序列的系统发育分析表明，菌株ACCC 02193属于欧文氏菌属，与塔斯马尼亚欧文氏菌DSM 17950（99.32%）和比氏欧文氏菌DSM 17872（98.77%）具有最高的相似性。然而，全基因组分析清楚地表明，菌株ACCC 02193与叶欧文氏菌CMYE1关系最为密切。该菌株在28℃-30℃下生长最佳，NaCl浓度为0-6%（w/v）（最适浓度为5%），pH值范围为4.0-10.0（最适pH值为8.0）。鉴定出的甲基萘醌为Q-8。主要脂肪酸（>10.0%）为C、C环、总和特征3（Cω7c和/或Cω6c）和总和特征8（Cω7c和/或Cω6c）。基因组比较表明，该菌株与密切相关的模式菌株的平均核苷酸同一性（ANI）≤82.8%，计算机模拟DNA-DNA杂交（DDH）值≤27.2%，这支持了我们的结论，即ACCC 02193代表欧文氏菌属中的一个新物种。该菌株的DNA G+C含量为55.7%。基于生理生化特征、基因型数据以及基因组比较的结果，我们证明模式菌株ACCC 02193（=GDMCC 1.4563=JCM 37069）是欧文氏菌属中的一个新物种，我们提议将其命名为气生欧文氏菌（Erwinia aeris sp. nov.）。
