Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio, USA.
Department of Specialty Medicine, College of Veterinary Medicine, Midwestern University, Glendale, Arizona, USA.
J Vet Intern Med. 2023 Mar;37(2):476-483. doi: 10.1111/jvim.16640. Epub 2023 Feb 7.
Serum 25-hydroxyvitamin (OH)D, C-reactive protein (CRP), and haptoglobin are useful biomarkers in various infectious diseases and inflammatory disorders in dogs, but their utility in histoplasmosis is unknown.
Determine if serum 25(OH)D, CRP, and haptoglobin concentrations are different in dogs with histoplasmosis compared to healthy controls and whether serum globulin, albumin, CRP, or haptoglobin are associated with 25(OH)D concentration.
Twenty-two client-owned dogs (histoplasmosis, n = 12; controls, n = 10).
Prospective case-control study. Dogs with histoplasmosis were categorized as pulmonary, disseminated, or gastrointestinal (GI) tract. Serum 25(OH)D was measured using modified high-performance liquid chromatography (HPLC). Serum CRP and haptoglobin were measured with ELISA assays.
Dogs with histoplasmosis were grouped as disseminated (n = 8) and GI tract (n = 4). No dogs had pulmonary tract involvement alone. Dogs with histoplasmosis (median, interquartile range [IQR]; 11.6 ng/mL, 16.8) had lower serum 25(OH)D concentrations than controls (35.7 ng/mL, 17.6; P < .001). Serum CRP and haptoglobin concentrations were higher in dogs with histoplasmosis (CRP: median, IQR; 63.5 mg/L, 37.1 and haptoglobin: 459.7 mg/dL, 419.6) than controls (CRP: 1.9 mg/L, 2; P < .001 and haptoglobin: 85.5 mg/dL, 106.7; P = .003). Serum 25(OH)D concentration was positively associated with fold change in serum albumin concentration (ρ = 0.77; P < .001), and negatively associated with fold change in serum globulin (ρ = -0.61; P = .003) and CRP concentrations (ρ = -0.56; P = .01).
Assay of serum 25(OH)D, CRP, and haptoglobin could have clinical value in dogs with histoplasmosis.
血清 25-羟维生素（OH）D、C 反应蛋白（CRP）和触珠蛋白是犬各种感染性疾病和炎症性疾病的有用生物标志物，但它们在组织胞浆菌病中的应用尚不清楚。
确定患有组织胞浆菌病的犬与健康对照组相比，血清 25（OH）D、CRP 和触珠蛋白的浓度是否不同，以及血清球蛋白、白蛋白、CRP 或触珠蛋白是否与 25（OH）D 浓度相关。
22 只患犬（组织胞浆菌病，n=12；对照组，n=10）。
前瞻性病例对照研究。患有组织胞浆菌病的犬分为肺部、播散性或胃肠道（GI）道。使用改良高效液相色谱法（HPLC）测量血清 25（OH）D。采用酶联免疫吸附试验（ELISA）检测血清 CRP 和触珠蛋白。
患有组织胞浆菌病的犬分为播散性（n=8）和胃肠道（n=4）两组。没有犬单独出现肺部受累。患有组织胞浆菌病的犬（中位数，四分位距[IQR]；11.6ng/mL，16.8）的血清 25（OH）D 浓度低于对照组（35.7ng/mL，17.6；P<0.001）。患有组织胞浆菌病的犬的血清 CRP 和触珠蛋白浓度高于对照组（CRP：中位数，IQR；63.5mg/L，37.1 和触珠蛋白：459.7mg/dL，419.6）（CRP：1.9mg/L，2；P<0.001 和触珠蛋白：85.5mg/dL，106.7；P=0.003）。血清 25（OH）D 浓度与血清白蛋白浓度的变化呈正相关（ρ=0.77；P<0.001），与血清球蛋白（ρ=-0.61；P=0.003）和 CRP 浓度（ρ=-0.56；P=0.01）的变化呈负相关。
血清 25（OH）D、CRP 和触珠蛋白的检测可能对患有组织胞浆菌病的犬具有临床价值。
