Zhang Jinxiu, Shui Xiaoxi, Jia Lifang, Wang Xueqi, Lv Jianhua, Wang Li-An
College of Life Science, Hebei Normal University, Shijiazhuang, 050024, P.R. China.
College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, People's Republic of China.
Int J Med Mushrooms. 2023;25(2):67-75. doi: 10.1615/IntJMedMushrooms.2022046817.
Box Behnken design (BBD) was used to optimize the extraction of Paxillus involutus (EPI) in ethanol. The optimum extraction conditions were as follows: temperature 45°C; solid:liquid ratio 1:35; time 5 h. Under these conditions, the yield of EPI was 13.57%. The antioxidant activity of EPI was evaluated in vitro, and DPPH free radical scavenging, ABTS free radical scavenging, and hydroxyl free radical scavenging effects were found to be equal to or close to that of the positive control vitamin C (VC). The antioxidant activity of EPI was next evaluated in vivo using aging mice; it was found to have appreciable effect on scavenging malonic dialdehyde (MDA) and could increase the activity of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT), and total superoxide dismutase (T-SOD) in mice. Overall, EPI showed antioxidant effects in aging mice, thereby delaying aging, and has potential for application as a natural antioxidant and in medical applications.
采用Box-Behnken设计（BBD）对卷缘桩菇乙醇提取物（EPI）的提取工艺进行优化。最佳提取条件如下：温度45℃；料液比1:35；时间5小时。在此条件下，EPI的得率为13.57%。体外评价了EPI的抗氧化活性，发现其对DPPH自由基、ABTS自由基和羟自由基的清除效果与阳性对照维生素C（VC）相当或接近。接下来，利用衰老小鼠对EPI的抗氧化活性进行体内评价；结果发现，EPI对清除丙二醛（MDA）有显著作用，且能提高小鼠体内谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和总超氧化物歧化酶（T-SOD）的活性。总体而言，EPI在衰老小鼠中表现出抗氧化作用，从而延缓衰老，具有作为天然抗氧化剂及用于医学应用的潜力。
