School of Stomatology, Zunyi Medical University, Zunyi, China.
Department of Endodontics, Guiyang Stomatological Hospital, 253 Jiefang Road, Nanming District, Guiyang, 550005, Guizhou, China.
Clin Oral Investig. 2023 Jun;27(6):2843-2849. doi: 10.1007/s00784-023-04874-x. Epub 2023 Feb 7.
The purpose of this study was to investigate whether there is a causal relationship between periodontitis and breast cancer by Mendelian randomization analysis.
We performed a two-sample bidirectional Mendelian randomization (MR) analysis using publicly released genome-wide association studies (GWAS) statistics. The inverse-variance weighted (IVW) method was used as the primary analysis. We applied complementary methods, including weighted median, weighted mode, simple mode, MR-Egger regression, and MR-pleiotropy residual sum and outlier (MR-PRESSO) to detect and correct for the effect of horizontal pleiotropy.
IVW MR analysis showed no effect of periodontitis on breast cancer (IVW OR=0.99, P =0.14). Similarly, no significant causal relationship between breast cancer and periodontitis was found in reverse MR analysis (IVW OR=0.95, P =0.83). The results of MR-Egger regression, weighted median, and weighted mode methods were consistent with those of the IVW method. Based on sensitivity analyses, horizontal pleiotropy is unlikely to distort causal estimates.
Although observational studies have reported an association between periodontitis and breast cancer, the results of our MR analysis do not support a causal relationship between periodontitis and breast cancer.
Mendelian randomization study can more clearly analyze the causal relationship between periodontitis and breast cancer, in order to provide a certain reference for clinicians and deepen the understanding of the relationship between periodontitis and breast cancer, to explore more possible associations between periodontitis and systemic diseases.
本研究旨在通过孟德尔随机化分析来探究牙周炎与乳腺癌之间是否存在因果关系。
我们利用公开发布的全基因组关联研究（GWAS）统计数据进行了两样本双向孟德尔随机化（MR）分析。采用逆方差加权（IVW）法作为主要分析方法。我们还应用了补充方法，包括加权中位数、加权众数、简单众数、MR-Egger 回归和 MR 偏倚剩余总和和异常值（MR-PRESSO），以检测和校正水平偏倚的影响。
IVW MR 分析显示，牙周炎对乳腺癌没有影响（IVW OR=0.99，P=0.14）。同样，反向 MR 分析也未发现乳腺癌与牙周炎之间存在显著的因果关系（IVW OR=0.95，P=0.83）。MR-Egger 回归、加权中位数和加权众数方法的结果与 IVW 方法一致。基于敏感性分析，水平偏倚不太可能扭曲因果估计。
尽管观察性研究报告了牙周炎与乳腺癌之间的关联，但我们的 MR 分析结果并不支持牙周炎与乳腺癌之间存在因果关系。
孟德尔随机化研究可以更清楚地分析牙周炎与乳腺癌之间的因果关系，以便为临床医生提供一定的参考，并加深对牙周炎与乳腺癌之间关系的理解，以探索牙周炎与系统性疾病之间可能存在的更多关联。
