Zhang Xuan-Qi, Xamxidin Maripat, Li Jing-Hang, Zhang Xin-Yin, Wang Tao, Chen Can, Wu Min, Ke Han
College of Civil Engineering and Architecture, Zhejiang University, Hangzhou 310058, PR China.
College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China.
Int J Syst Evol Microbiol. 2023 Feb;73(2). doi: 10.1099/ijsem.0.005685.
Bacterial strain Y-6, isolated from a landfill site in Yiwu, PR China, was characterized using a polyphasic taxonomy approach. Cells were Gram-stain-negative, aerobic, rod-shaped, motile by means of a single polar flagellum and formed pale beige colonies. Strain Y-6 grew at 4-40 °C (optimal at 30-37 °C), pH 6.5-9.5 (optimal at pH 7.2-8.5) and in the presence of 0.5-10.0 % (w/v) NaCl (optimal at 1.0-3.0 %). Phylogenetic analysis revealed that strain Y-6 was a member of the genus and closely related to MCCC 1A06493 with a 16S rRNA sequence similarity of 98.2 %. The major cellular fatty acids of the isolate were iso-C, C, iso-C and summed feature 9 (iso-C 9 and/or 10-methyl-C). Q-8 was the predominant ubiquinone. The major polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, aminoglycophospholipid, aminophospholipid, phospholipid, three glycolipids and two unknown lipids. The genomic DNA G+C content was 46.6 mol%. The digital DNA-DNA hybridization value between Y-6 and A. taiwanensis MCCC 1A06493 was 18.3 %. Strain Y-6 had an average nucleotide identity value of 74.09 % with A. taiwanensis MCCC 1A06493. Results from the polyphasic taxonomy study support the conclusion that strain Y-6 represents a novel species, for which the name sp.nov. is proposed. The type strain is Y-6 (=MCCC 1K06228=KCTC 82676).
从中国浙江省义乌市的一个垃圾填埋场分离出的细菌菌株Y-6,采用多相分类法进行了表征。细胞革兰氏染色阴性,好氧,杆状,通过单根极生鞭毛运动,形成浅米色菌落。菌株Y-6在4-40°C(最适温度为30-37°C)、pH 6.5-9.5(最适pH为7.2-8.5)以及0.5-10.0%(w/v)NaCl(最适浓度为1.0-3.0%)的条件下生长。系统发育分析表明,菌株Y-6是该属的一个成员,与MCCC 1A06493密切相关,16S rRNA序列相似性为98.2%。该分离株的主要细胞脂肪酸为异-C、C、异-C和总和特征9(异-C 9和/或10-甲基-C)。泛醌Q-8是主要的泛醌。主要极性脂类包括双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、氨基糖磷脂、氨基磷脂、磷脂、三种糖脂和两种未知脂类。基因组DNA的G+C含量为46.6mol%。Y-6与台湾嗜盐放线菌MCCC 1A06493之间的数字DNA-DNA杂交值为18.3%。菌株Y-6与台湾嗜盐放线菌MCCC 1A06493的平均核苷酸同一性值为74.09%。多相分类研究结果支持菌株Y-6代表一个新物种的结论,为此提出新物种名称sp.nov.。模式菌株为Y-6(=MCCC 1K( )6228=KCTC 82676)。 (注:原文中部分括号内容缺失,已按原样翻译)