Zhang Lei, Zhang Baojian, Liao Boyu, Yuan Sha, Liu Yanqun, Liao Zhengyin, Cheng Biao
Department of Plastic Surgery, General Hospital of Southern Theater Command, PLA Guangzhou 510010, China.
Institute of Huabo Biopharmaceutical Guangzhou 510010, China.
Am J Transl Res. 2019 Jul 15;11(7):4100-4112. eCollection 2019.
Adipose-derived stem cells (ADSCs) are multipotent stromal cells that provide an abundant source of cells for skin tissue engineering and wound healing. Platelet-rich plasma (PRP) is a concentrate of platelet-rich plasma protein, which contains several different growth factors and other cytokines. In this study, we combined ADSCs with PRP for wound healing. Herein, we found ADSCs in combination with PRP was able to promote wound healing, granulation formation, collagen deposition and re-epithelialization. The mechanism exploration discovered that PRP promoted stress fiber formation in ADSCs, leading to cell migration. Then, we demonstrated that PRP enhanced the expression of Rho GTP family proteins, including Cdc 42, Rac 1 and Rho A. Moreover, it promoted the expression of downstream Rho GTP signaling molecules, including PAK 1, ROCK 2, LIMK 1 and Cofilin. When PRP was used in combination with the Cdc 42 inhibitor ZCL278, the Rho A inhibitor CT04, Rac 1 inhibitor NSC23766, PAK inhibitor FRAX597, or Rock 2 inhibitor Y27632 to treat ADSCs, stress fiber formation was significantly reduced, resulting in decreased cell migration. Our findings may provide a promising approach to promote wound healing.
脂肪来源干细胞（ADSCs）是多能间充质细胞，为皮肤组织工程和伤口愈合提供了丰富的细胞来源。富血小板血浆（PRP）是富含血小板血浆蛋白的浓缩物，其中含有几种不同的生长因子和其他细胞因子。在本研究中，我们将ADSCs与PRP联合用于伤口愈合。在此，我们发现ADSCs与PRP联合能够促进伤口愈合、肉芽形成、胶原蛋白沉积和再上皮化。机制探索发现，PRP促进了ADSCs中应力纤维的形成，导致细胞迁移。然后，我们证明PRP增强了Rho GTP家族蛋白的表达，包括Cdc 42、Rac 1和Rho A。此外，它促进了下游Rho GTP信号分子的表达，包括PAK 1、ROCK 2、LIMK 1和Cofilin。当PRP与Cdc 42抑制剂ZCL278、Rho A抑制剂CT04、Rac 1抑制剂NSC23766、PAK抑制剂FRAX597或Rock 2抑制剂Y27632联合用于处理ADSCs时，应力纤维的形成显著减少，导致细胞迁移减少。我们的研究结果可能为促进伤口愈合提供一种有前景的方法。
