Cai Ming, Zhu Hua, Xu Lei, Wang Jian, Xu Jing, Li Zhenhao, Yang Kai, Wu Jianyong, Sun Peilong
Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, People's Republic of China; Key Laboratory of Food Macromolecular Resources Processing Technology Research (Zhejiang University of Technology), China National Light Industry, People's Republic of China.
Department of Food Science and Technology, Zhejiang University of Technology, Hangzhou, Zhejiang 310014, People's Republic of China; Key Laboratory of Food Macromolecular Resources Processing Technology Research (Zhejiang University of Technology), China National Light Industry, People's Republic of China.
Int J Biol Macromol. 2023 Apr 15;234:123572. doi: 10.1016/j.ijbiomac.2023.123572. Epub 2023 Feb 6.
This study was to investigate the antifatigue, prebiotic effects and their relationships to the structure properties of three ethanol precipitated polysaccharides from Dendrobium officinale (EPDO), as EPDO-40, EPDO-60 and EPDO-80. EPDOs with anti-fatigue activity were screened out by forced swimming test, and blood lactic acid (BLA), blood urea nitrogen (BUN), superoxide dismutase (SOD), liver glycogen, muscle glycogen, and intestinal microflora were investigated. Results showed that purified EPDO-60, 277.3 kDa, with a backbone consisted of 4-Manp and 4-Glcp. EPDO-60 had the best anti-fatigue activity, because it could significantly prolong the forced swimming time, as well as down-regulating the levels of BLA and BUN, increasing SOD. Proportions of Bacteroidetes and Firmicutes and abundance of Lactobacillus and Bifidobacterium in gut microflora increased after treated with EPDO-60. Accordingly, EPDO-60 could affect the community structure of gut microflora, leading to promote the balance of oxidation and antioxidation, and accelerated the fatigue metabolism in vivo.
本研究旨在探讨铁皮石斛三种乙醇沉淀多糖（EPDO），即EPDO - 40、EPDO - 60和EPDO - 80的抗疲劳、益生元作用及其与结构特性的关系。通过强迫游泳试验筛选出具有抗疲劳活性的EPDO，并对血乳酸（BLA）、血尿素氮（BUN）、超氧化物歧化酶（SOD）、肝糖原、肌糖原和肠道微生物群进行了研究。结果表明，纯化后的EPDO - 60分子量为277.3 kDa，主链由4 - 甘露糖和4 - 葡萄糖组成。EPDO - 60具有最佳的抗疲劳活性，因为它可以显著延长强迫游泳时间，同时下调BLA和BUN水平，提高SOD活性。用EPDO - 60处理后，肠道微生物群中拟杆菌门和厚壁菌门的比例以及乳酸杆菌和双歧杆菌的丰度增加。因此，EPDO - 60可以影响肠道微生物群的群落结构，促进体内氧化与抗氧化的平衡，并加速疲劳代谢。
