Department of Intensive Care Medicine, Eastern Hepatobiliary Surgery Hospital, The Third Affiliated Hospital of Naval Medical University, Shanghai, China.
Dis Markers. 2023 Jan 30;2023:3560340. doi: 10.1155/2023/3560340. eCollection 2023.
Differentially transcription factors (DETFs) were identified from differentially expressed genes (DEGs) in GSE62232 and transcription factors. Then, they were analyzed by regulatory networks, prognostic risk model, and overall survival analyses to identify the key DETF. Combined with the regulatory networks and binding site analysis, the target mRNA of key DETF was determined, and its prognostic value in HCC was evaluated by survival, clinical characteristics analyses, and experiments. Finally, the expressions and functions of the key DETF on the DEmRNAs were investigated in HCC cells.
Through multiple bioinformatics analyses, was identified as the key DETF, and was predicted to be its target mRNA with the common binding site of CCAGCAACTGGCC, both downregulated in HCC. In survival analysis, high was related to better HCC prognosis, and was differentially expressed in HCC patients with clinical characteristics. Furthermore, cell experiments showed the mRNA expressions of and were both reduced in HCC, and their overexpressions suppressed the growth, invasion, and migration of HCC cells. Besides, over- could upregulate expression in HCC cells.
This study identifies two suppressor genes in HCC progression, and , and the key transcription factor suppresses HCC progression by targeting mRNA. They are both potential treatment targets and prognostic biomarkers for HCC patients, which provides new clues for HCC research.
从 GSE62232 和转录因子中的差异表达基因 (DEG) 中鉴定差异转录因子 (DETF)。然后，通过调控网络、预后风险模型和总生存分析对其进行分析，以确定关键的 DETF。结合调控网络和结合位点分析，确定关键 DETFs 的靶 mRNA，并通过生存、临床特征分析和实验评估其在 HCC 中的预后价值。最后，在 HCC 细胞中研究关键 DETFs 对 DEmRNAs 的表达和功能。
通过多种生物信息学分析，确定为关键 DETF，预测为其靶 mRNA，具有常见的结合位点 CCAGCAACTGGCC，两者在 HCC 中均下调。在生存分析中，高表达与更好的 HCC 预后相关，在具有临床特征的 HCC 患者中存在差异表达。此外，细胞实验表明 HCC 中 和 的 mRNA 表达均降低，过表达抑制 HCC 细胞的生长、侵袭和迁移。此外，过表达可以上调 HCC 细胞中 的表达。
本研究鉴定了 HCC 进展中的两个抑制基因 和 ，关键转录因子 通过靶向 mRNA 抑制 HCC 进展。它们都是 HCC 患者潜在的治疗靶点和预后生物标志物，为 HCC 研究提供了新的线索。
