Chelly Sabrine, Chelly Meryam, Zribi Rayhane, Gdoura Radhouane, Bouaziz-Ketata Hanen, Neri Giovanni
Laboratory of Toxicology-Microbiology Environmental and Health, LR17ES06, Sfax Faculty of Sciences, University of Sfax, BP 1171, 3000 Sfax, Tunisia.
Department of Engineering, University of Messina, C.Da Di Dio, I-98166 Messina, Italy.
ACS Omega. 2021 Sep 9;6(37):23666-23675. doi: 10.1021/acsomega.1c00793. eCollection 2021 Sep 21.
A AuNP-modified screen-printed carbon electrode (AuNP/SPCE) for monitoring important biomolecules, such as dopamine (DA) and riboflavin (RF), having a high potential for personalized medicine and for continuous monitoring of human health is here proposed. AuNPs were synthesized using the extract of as a reducing medium and were characterized by UV-vis spectroscopy, dynamic light scattering (DLS), and scanning and transmission electron microscopy (SEM and TEM). The synthesized AuNPs appear spherical and present a bimodal size distribution with a maximum centered at around 30-50 nm. Cyclic voltammetry (CV) experiments demonstrated that the modified AuNP/SPCE sensor exhibits superior electrochemical performances to the bare SPCE. Low limits of detection (LODs) of 0.2 and 0.07 μM at S/N = 3 and sensitivities of 550.4 and 2399 μA mM cm were registered for DA and RF detection, respectively. Results demonstrate the promising electrochemical characteristics of the synthesized AuNPs and developed AuNP/SPCE electrochemical sensor for the determination of these important biomolecules.
本文提出了一种用于监测多巴胺（DA）和核黄素（RF）等重要生物分子的金纳米颗粒修饰丝网印刷碳电极（AuNP/SPCE），该电极在个性化医疗和人体健康连续监测方面具有巨大潜力。使用[提取物名称]提取物作为还原介质合成了金纳米颗粒，并通过紫外可见光谱、动态光散射（DLS）以及扫描和透射电子显微镜（SEM和TEM）对其进行了表征。合成的金纳米颗粒呈球形，呈现双峰尺寸分布，最大值集中在30 - 50 nm左右。循环伏安法（CV）实验表明，修饰后的AuNP/SPCE传感器表现出优于裸SPCE的电化学性能。在信噪比（S/N） = 3时，DA和RF检测的检测限（LOD）分别低至0.2和0.07 μM，灵敏度分别为550.4和2399 μA mM⁻¹ cm⁻²。结果表明，合成的金纳米颗粒和开发的用于测定这些重要生物分子的AuNP/SPCE电化学传感器具有良好的电化学特性。
