Department of Chemistry, SRM Institute of Science and Technology, Kattankulathur-603203, Tamil Nadu, India.
Anal Methods. 2023 Nov 2;15(42):5639-5654. doi: 10.1039/d3ay01493b.
In this work, the crystal plane of strontium oxide (SrO) nanorods was integrated into hexagonal-boron nitride (h-BN) nanosheets to form 1D-2D (SrO/h-BN) composite were utilized for the electrochemical detection of the chemotherapeutic drug 5-fluorouracil (5-Fu). 5-Fu is a clinically proven and the third most frequently applied chemotherapeutic drug for treating solid tumours, such as colorectal, stomach, cutaneous and breast malignancies. Its overdoses lead to toxic metabolite accumulation that has serious adverse consequences on humans, including neurotoxicity, death and the induction of morbidity. Therefore, to improve the chemotherapy and predict the potential adverse effects of 5-Fu residues in the human body, susceptible and quick analytical methods for detecting 5-Fu in human body fluids (blood serum/plasma and urine) are needed. The effective interaction of the synthesized SrO/h-BN composite shows increased efficiency for the electrochemical detection of 5-Fu with good selectivity. Notably, a simple sonochemical method achieved a synergistic interaction between the (100) plane of SrO and the (002) plane of h-BN. Various analytical and spectroscopic techniques were used to characterize the SrO/h-BN nanocomposite, which provided useful insights into the composition and properties of the composite material. The crystalline, structural and chemical characteristics of the as-synthesized material were characterized by XRD, Raman spectroscopy, HR-TEM, XPS and HR-SEM. Furthermore, the proposed electrode's electrochemical sensing capability was analysed using CV, EIS, DPV and - curve methods. Numerous active sites created on a modified electrode enhanced the mass transport and electron transfer rate, thereby increasing the electrochemical activity towards the 5-Fu detection. Consequently, under optimized conditions, the SrO/h-BN/GCE exhibited remarkable selectivity, durability, low detection limit (0.003 μM) and wide linear range (0.02-56 μM) for 5-Fu. Finally, the successful application of this sensor for 5-Fu detection in biological samples was successfully tested with high recovery percentages.
在这项工作中，将氧化锶（SrO）纳米棒的晶面整合到六方氮化硼（h-BN）纳米片中，形成 1D-2D（SrO/h-BN）复合材料，用于电化学检测化疗药物 5-氟尿嘧啶（5-Fu）。5-Fu 是一种经过临床验证的、第三大常用化疗药物，用于治疗结直肠、胃、皮肤和乳腺恶性肿瘤等实体瘤。其过量会导致有毒代谢物的积累，对人体产生严重的不良后果，包括神经毒性、死亡和发病率的诱导。因此，为了改善化疗效果并预测 5-Fu 在人体内的潜在不良反应，需要开发用于检测人体体液（血清/血浆和尿液）中 5-Fu 的灵敏、快速的分析方法。所合成的 SrO/h-BN 复合材料的有效相互作用显示出对 5-Fu 的电化学检测具有更高的效率和良好的选择性。值得注意的是，一种简单的超声化学方法实现了 SrO 的（100）面和 h-BN 的（002）面之间的协同相互作用。使用各种分析和光谱技术对 SrO/h-BN 纳米复合材料进行了表征，为复合材料的组成和性质提供了有用的见解。通过 XRD、拉曼光谱、高分辨率透射电子显微镜（HR-TEM）、X 射线光电子能谱（XPS）和高分辨率扫描电子显微镜（HR-SEM）对合成材料的晶体、结构和化学特性进行了表征。此外，还通过 CV、EIS、DPV 和 I-V 曲线方法对所提出电极的电化学传感能力进行了分析。修饰电极上产生的大量活性位点增强了质量传输和电子转移速率，从而提高了对 5-Fu 检测的电化学活性。因此，在优化条件下，SrO/h-BN/GCE 对 5-Fu 表现出显著的选择性、耐用性、低检测限（0.003 μM）和宽线性范围（0.02-56 μM）。最后，该传感器成功地应用于生物样品中 5-Fu 的检测，具有较高的回收率。
