尺寸和成分依赖性道路灰尘对人结肠细胞和类器官的毒性诱导作用。
Induction of toxicity in human colon cells and organoids by size- and composition-dependent road dust.
作者信息
Park Sung Bum, Kim Eun-Ah, Kim Ki Young, Koh Byumseok
机构信息
Biotechnology and Therapeutics Division, Korea Research Institute of Chemical Technology 141 Gajeong-ro, Yuseong-gu Daejeon 34114 Republic of Korea
National Assembly Futures Institute Members Office Bldg, 1 Uisadang-daero, Yeongdeungpo-gu Seoul 07233 Republic of Korea.
出版信息
RSC Adv. 2023 Jan 18;13(5):2833-2840. doi: 10.1039/d2ra07500h.
Environmental pollution, including the annual resurgence of particulate matter derived from road dust, is a serious issue worldwide. Typically, the size of road dust is less than 10 μm; thus, road dust can penetrate into human organs, including the brain, through inhalation and intake by mouth. Therefore, the toxicity of road dust has been intensively studied and . However, systems, including 2D cell cultures, cannot mimic complex human organs, and there are several discrepancies between and human systems. Here, we used human colon cells and organoids to evaluate the cytotoxicity of particulate matter derived from road dust. The toxicity of road dust collected in industrialized and high traffic areas and NIST urban particulate matter reference samples were evaluated in 2D and 3D human colon cells as well as colon organoids and their characteristics were carefully examined. Data suggest that the size and elemental compositions of road dust can correlate with colon organoid toxicity, and thus, a more careful assessment of the size and elemental compositions of road dust should be conducted to predict its effect on human health.
环境污染，包括每年都会重新出现的源自道路扬尘的颗粒物，是一个全球性的严重问题。通常，道路扬尘的粒径小于10微米；因此，道路扬尘可通过吸入和经口摄入进入人体器官，包括大脑。因此，道路扬尘的毒性已得到深入研究。然而，包括二维细胞培养在内的系统无法模拟复杂的人体器官，而且体外系统与人体系统之间存在一些差异。在这里，我们使用人类结肠细胞和类器官来评估源自道路扬尘的颗粒物的细胞毒性。对在工业化和交通繁忙地区收集的道路扬尘以及美国国家标准与技术研究院（NIST）城市颗粒物参考样品的毒性在二维和三维人类结肠细胞以及结肠类器官中进行了评估，并仔细检查了它们的特性。数据表明，道路扬尘的粒径和元素组成可能与结肠类器官毒性相关，因此，应该对道路扬尘的粒径和元素组成进行更仔细的评估，以预测其对人类健康的影响。
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