Peng Lin, Liang Yufei, Yue Jianling, Li Hanmei, Deng Aimin, Xie Shun, Tang Xiu-Zhi, Wang Jing, Mao Zenghui
Changsha Hospital for Maternal and Child Health Care Affiliated to Hunan Normal University Changsha 410083 China
Powder Metallurgy Research Institute, Central South University Changsha 410083 China.
RSC Adv. 2023 Jan 25;13(6):3635-3642. doi: 10.1039/d2ra07622e. eCollection 2023 Jan 24.
Human amniotic membrane (hAM) is a promising material for tissue engineering due to several benefits, including desirable biocompatibility, stem cell source, antibacterial activity, . However, because of its low elasticity, the clinical application of hAM is severely restricted. To solve this issue, we employed polydopamine/polyacrylamide (PDA/PAM) hydrogels to toughen hAM. The test results indicated that the PDA/PAM hydrogel can enhance the toughness of hAM dramatically due to the formation of abundant chemical bonds and the strong mechanical properties of the hydrogel itself. Compared to pure hAM, the break elongation and tensile strength of PDA/PAM-toughened hAM rose by 154.15 and 492.31%, respectively. And most importantly, the fracture toughness was almost 15 times higher than untreated hAM. In addition, the cytotoxicity of the PDA/PAM-coated hAM was not detected due to the superior biocompatibility of the chemicals used in the study. Treating hAM with adhesive hydrogels to increase its mechanical characteristics will further promote the application of hAM as a tissue engineering material.
人羊膜（hAM）因其多种优势，包括良好的生物相容性、干细胞来源、抗菌活性等，是一种很有前景的组织工程材料。然而，由于其弹性较低，hAM的临床应用受到严重限制。为了解决这个问题，我们采用聚多巴胺/聚丙烯酰胺（PDA/PAM）水凝胶来增强hAM的韧性。测试结果表明，PDA/PAM水凝胶能够显著提高hAM的韧性，这是由于形成了大量化学键以及水凝胶本身具有强大的机械性能。与纯hAM相比，PDA/PAM增强的hAM的断裂伸长率和拉伸强度分别提高了154.15%和492.31%。最重要的是，其断裂韧性几乎比未处理的hAM高15倍。此外，由于研究中使用的化学物质具有优异的生物相容性，未检测到PDA/PAM涂层hAM的细胞毒性。用粘性水凝胶处理hAM以提高其机械性能将进一步推动hAM作为组织工程材料的应用。
